Involvement of Rho-kinase in sphingosine 1-phosphate-stimulated HSP27 induction in osteoblasts

  • Authors:
    • Chiho Minamitani
    • Takanobu Otsuka
    • Shinji Takai
    • Rie Matsushima-Nishiwaki
    • Seiji Adachi
    • Yoshiteru Hanai
    • Jun Mizutani
    • Haruhiko Tokuda
    • Osamu Kozawa
  • View Affiliations

  • Published online on: July 1, 2009     https://doi.org/10.3892/ijmm_00000209
  • Pages: 77-82
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Abstract

We previously reported that sphingosine 1-phosphate induces heat shock protein 27 (HSP 27) via activation of phosphatidylinositol 3-kinase (PI3K)/Akt and p38 mitogen-activated protein (MAP) kinase in osteoblast-like MC3T3-E1 cells. In the present study, we investigated whether Rho-kinase is implicated in sphingosine 1-phosphate-stimulated induction of HSP27 in MC3T3-E1 cells. Sphingosine 1-phosphate time-dependently induced the phosphorylation of myosin phosphatase targeting subunit (MYPT-1), a Rho-kinase substrate. Y27632, a specific Rho-kinase inhibitor, significantly reduced sphingosine 1-phosphate-stimulated HSP27 induction, as well as MYPT-1 phosphorylation. Fasudil, another inhibitor of Rho-kinase, also suppressed sphingosine 1-phosphate-stimulated HSP27 induction. Y27632, as well as fasudil, attenuated sphingosine 1-phosphate-induced phosphorylation of p38 MAP kinase. However, Akt phosphorylation induced by sphingosine 1-phosphate was not affected by either Rho-kinase inhibitor. These results strongly suggest that Rho-kinase regulates sphingosine 1-phosphate-stimulated induction of HSP27 at a point upstream of p38 MAP kinase in osteoblasts.

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July 2009
Volume 24 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Minamitani C, Otsuka T, Takai S, Matsushima-Nishiwaki R, Adachi S, Hanai Y, Mizutani J, Tokuda H and Kozawa O: Involvement of Rho-kinase in sphingosine 1-phosphate-stimulated HSP27 induction in osteoblasts. Int J Mol Med 24: 77-82, 2009
APA
Minamitani, C., Otsuka, T., Takai, S., Matsushima-Nishiwaki, R., Adachi, S., Hanai, Y. ... Kozawa, O. (2009). Involvement of Rho-kinase in sphingosine 1-phosphate-stimulated HSP27 induction in osteoblasts. International Journal of Molecular Medicine, 24, 77-82. https://doi.org/10.3892/ijmm_00000209
MLA
Minamitani, C., Otsuka, T., Takai, S., Matsushima-Nishiwaki, R., Adachi, S., Hanai, Y., Mizutani, J., Tokuda, H., Kozawa, O."Involvement of Rho-kinase in sphingosine 1-phosphate-stimulated HSP27 induction in osteoblasts". International Journal of Molecular Medicine 24.1 (2009): 77-82.
Chicago
Minamitani, C., Otsuka, T., Takai, S., Matsushima-Nishiwaki, R., Adachi, S., Hanai, Y., Mizutani, J., Tokuda, H., Kozawa, O."Involvement of Rho-kinase in sphingosine 1-phosphate-stimulated HSP27 induction in osteoblasts". International Journal of Molecular Medicine 24, no. 1 (2009): 77-82. https://doi.org/10.3892/ijmm_00000209