Activity of the 5-HT1A receptor is involved in the alteration of glucocorticoid receptor in hippocampus and corticotropin-releasing factor in hypothalamus in SPS rats

  • Authors:
    • Hai-Tao Wang
    • Fang Han
    • Yu-Xiu Shi
  • Corresponding author:
  • View Affiliations

  • Published online on: Saturday, August 1, 2009
  • Pages: 227-231 DOI: 10.3892/ijmm_00000225

Abstract

Rats exposed to single-prolonged stress (SPS) showed enhanced inhibition of the hypothalamic-pituitary-adrenal (HPA) system and alteration in the glucocorticoid/mineralocorticoid receptor. Dysfuntion of the HPA axis is one of the core neuroendocrine abnormalities of post-traumatic stress disorder (PTSD). Serotonergic receptor, glucocorticoid receptor (GR) and corticotropin-releasing factor (CRF) have been proposed to play major roles in dysfuntion of the HPA axis. However, the precise molecular mechanism is unknown. In this study, we investigated the relationships between the changes of GR in hippocampus as well as CRF in hypothalamus and the activity of 5-HT1A receptor in SPS rats. We exposed rats to SPS with or without prior treatment with WAY100635 (the 5-HT1A receptor antagonist), and observed behavioral changes, GR levels in the hippocampus and CRF levels in the hypothalamus by immunohistochemistry, Western blotting and RT-PCR seven days after SPS. Our results demonstrate that SPS increases expression of GR and CRF, which were partially inhibited by WAY-100635.
Journal Cover

August 2009
Volume 24 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

2013 Impact Factor: 1.88
2014 I.F. (Expected) ≥ 2.302 Ranked #27/122 Medicine Research and Experimental
(total number of cites)

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APA
Wang, H., Han, F., & Shi, Y. (2009). Activity of the 5-HT1A receptor is involved in the alteration of glucocorticoid receptor in hippocampus and corticotropin-releasing factor in hypothalamus in SPS rats. International Journal of Molecular Medicine, 24, 227-231. http://dx.doi.org/10.3892/ijmm_00000225
MLA
Wang, H., Han, F., Shi, Y."Activity of the 5-HT1A receptor is involved in the alteration of glucocorticoid receptor in hippocampus and corticotropin-releasing factor in hypothalamus in SPS rats". International Journal of Molecular Medicine 24.2 (2009): 227-231.
Chicago
Wang, H., Han, F., Shi, Y."Activity of the 5-HT1A receptor is involved in the alteration of glucocorticoid receptor in hippocampus and corticotropin-releasing factor in hypothalamus in SPS rats". International Journal of Molecular Medicine 24, no. 2 (2009): 227-231. http://dx.doi.org/10.3892/ijmm_00000225