Induction of G1 arrest and apoptosis by schisandrin C isolated from Schizandra chinensis Baill in human leukemia U937 cells

  • Authors:
    • Cheol Park
    • Young-Whan Choi
    • Sook Kyung Hyun
    • Hyun Ju Kwon
    • Hye Jin Hwang
    • Gi-Young Kim
    • Byung Tae Choi
    • Byung-Woo Kim
    • Il-Whan Choi
    • Sung-Kwon Moon
    • Wun-Jae Kim
    • Yung Hyun Choi
  • View Affiliations

  • Published online on: Thursday, October 1, 2009
  • Pages: 495-502
  • DOI: 10.3892/ijmm_00000258

Abstract

We isolated two phytochemical lignans, schisandrin and schisandrin C, from Schizandra chinensis Baill and investigated their anti-cancer effects in human leukemia U937 cells. Schisandrin C inhibited cell growth in a dose-dependent manner, which was associated with the induction of G1 arrest of the cell cycle and apoptosis; schisandrin did not inhibit growth. Schisandrin C induced G1 arrest was correlated with down-regulation of cyclin D1, cyclin E, cyclin-dependent kinase (Cdk) 4 and E2Fs expression, inhibition of phosphorylation of retinoblastoma protein (pRB), and up-regulation of the Cdk inhibitor p21(WAF1/CIP1). In addition, schisandrin C-induced apoptosis was associated with down-regulation of expression of the anti-apoptotic proteins Bcl-2 and Bcl-xL, proteolytic activation of caspase-3 and -9, and a concomitant degradation of poly(ADP-ribose) polymerase (PARP). Furthermore, schisandrin C-induced apoptosis was significantly inhibited by a caspase-3 specific inhibitor z-DEVD-fmk, indicating an important role for caspase-3 in the schisandrin C mechanism. In summary, growth inhibition by schisandrin C is related to cell cycle arrest at G1 and induction of caspase-3-dependent apoptosis in U937 cells; these findings suggest that schisandrin C may be a useful chemotherapeutic agent.
Journal Cover

October 2009
Volume 24 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

2013 Impact Factor: 1.88
Ranked #27/122 Medicine Research and Experimental
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APA
Park, C., Choi, Y., Hyun, S., Kwon, H., Hwang, H., Kim, G., Choi, B., Kim, B., Choi, I., Moon, S., Kim, W., & Choi, Y. (2009). Induction of G1 arrest and apoptosis by schisandrin C isolated from Schizandra chinensis Baill in human leukemia U937 cells. International Journal of Molecular Medicine, 24(4), 495-502.
MLA
Park, Choi, Hyun, Kwon, Hwang, Kim, Choi, Kim, Choi, Moon, Kim, and Yung Choi. "Induction of G1 arrest and apoptosis by schisandrin C isolated from Schizandra chinensis Baill in human leukemia U937 cells." International Journal of Molecular Medicine International Journal of Molecular Medicine 24.4 (2009): 495-502.
Chicago
Park, Choi, Hyun, Kwon, Hwang, Kim, Choi, Kim, Choi, Moon, Kim, and Yung Choi. "Induction of G1 arrest and apoptosis by schisandrin C isolated from Schizandra chinensis Baill in human leukemia U937 cells." International Journal of Molecular Medicine International Journal of Molecular Medicine 24 no. 4 (2009): 495-502.