The effects of adiponectin on interleukin-6 and MCP-1 secretion in lipopolysaccharide-treated 3T3-L1 adipocytes: Role of the NF-κB pathway

  • Authors:
    • Elena Zoico
    • Ulisse Garbin
    • Debora Olioso
    • Gloria Mazzali
    • Anna M. Fratta Pasini
    • Vincenzo Di Francesco
    • Anna Sepe
    • Luciano Cominacini
    • Mauro Zamboni
  • Corresponding author:
  • View Affiliations

  • Published online on: Tuesday, December 1, 2009
  • Pages: 847-851 DOI: 10.3892/ijmm_00000302

Abstract

It was recently suggested that the transcription nuclear factor-κB (NF-κB) plays an important role in controlling the inflammation and metabolic alterations associated with obesity. In endothelial and monocytic cells, adiponectin acts as a modulator of the inflammatory response, suppressing NF-κB activation. The aim of this study was to assess the ability of different forms of adiponectin to modulate the inflammatory response in adipocytes. 3T3-L1 preadipocytes were cultured according to standard conditions. Fully differentiated adipocytes were stimulated with 1 µg/ml lipopolysaccharides (LPS) for 16 h, with or without pre-treatment with 10 µg/ml of globular (AdG) or full-length (AdFl) adiponectin. Both AdG and AdFl significantly suppressed LPS-induced expression of IL-6 mRNA in adipocytes and reduced the concentration of IL-6 in culture media. Adiponectin pre-treatment significantly reduced the increase in MCP-1 mRNA in adipocytes exposed to LPS. In culture media, the increase in MCP-1 detected after LPS stimulation was significantly attenuated after pre-treatment with AdG. In 3T3-L1, AdG and AdFl reduced NF-κB activity by 50 and 40%, respectively compared to the NF-κB activation induced by LPS alone. Moreover, both forms of adiponectin significantly attenuated IkB-α as well as IKK gene expression. Pre-treatment of adipocytes with AdG or AdFl significantly increased PPARγ mRNA levels, taking its expression back to the basal level. Both AdG and AdFl exert anti-inflammatory activity suppressing IL-6 and MCP-1 production from inflamed adipocytes. This anti-inflammatory action may be mediated through inhibition of NF-κB activity as well as through increased PPARγ expression.
Journal Cover

December 2009
Volume 24 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

2013 Impact Factor: 1.88
2014 I.F. (Expected) ≥ 2.372 Ranked #27/122 Medicine Research and Experimental
(total number of cites)

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APA
Zoico, E., Garbin, U., Olioso, D., Mazzali, G., Fratta Pasini, A.M., Di Francesco, V. ... Zamboni, M. (2009). The effects of adiponectin on interleukin-6 and MCP-1 secretion in lipopolysaccharide-treated 3T3-L1 adipocytes: Role of the NF-κB pathway . International Journal of Molecular Medicine, 24, 847-851. http://dx.doi.org/10.3892/ijmm_00000302
MLA
Zoico, E., Garbin, U., Olioso, D., Mazzali, G., Fratta Pasini, A. M., Di Francesco, V., Sepe, A., Cominacini, L., Zamboni, M."The effects of adiponectin on interleukin-6 and MCP-1 secretion in lipopolysaccharide-treated 3T3-L1 adipocytes: Role of the NF-κB pathway ". International Journal of Molecular Medicine 24.6 (2009): 847-851.
Chicago
Zoico, E., Garbin, U., Olioso, D., Mazzali, G., Fratta Pasini, A. M., Di Francesco, V., Sepe, A., Cominacini, L., Zamboni, M."The effects of adiponectin on interleukin-6 and MCP-1 secretion in lipopolysaccharide-treated 3T3-L1 adipocytes: Role of the NF-κB pathway ". International Journal of Molecular Medicine 24, no. 6 (2009): 847-851. http://dx.doi.org/10.3892/ijmm_00000302