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International Journal of Molecular Medicine
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Print ISSN: 1107-3756 Online ISSN: 1791-244X
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January 2010 Volume 25 Issue 1

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

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Article

Fabrication of a microarray using a combination of the large circular sense and antisense DNA

  • Authors:
    • Kyung-Oh Doh
    • Yun-Han Lee
    • Kil-Hwan Han
    • Seok-Yong Uhm
    • Jong-Pil Kim
    • Yun-Ui Bae
    • Jeong-Hoh Park
    • Ik-Jae Moon
    • Jong-Gu Park

  • View Affiliations / Copyright

    Affiliations: Department of Physiology, Yeungnam University School of Medicine, Daegu 705-717, Korea. kodoh@ynu.ac.kr
  • Pages: 113-120
    |
    Published online on: January 1, 2010
       https://doi.org/10.3892/ijmm_00000320
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Abstract

In the present study, single-stranded large circular (LC)-sense molecules were utilized as probes for DNA microarrays and showed stronger binding signals than those of PCR-amplified cDNA probes. A microarray experiment using 284 LC-sense DNA probes found 6 upregulated and 7 downregulated genes in A549 cells as compared to WI38VA13 cells. Repeated experiments showed largely consistent results, and microarray data strongly correlated with data acquired from quantitative real-time RT-PCR. A large array comprising 5,079 LC-sense DNA was prepared, and analysis of the mean differential expression from dye-swap experiments revealed 332 upregulated and 509 downregulated genes in A549 cells compared to WI38VA13 cells. Subsequent functional analysis using an LC-antisense library of overexpressed genes identified 28 genes involved in A549 cell growth. These experiments demonstrated the proper features of LC-sense molecules as probe DNA for microarray and the potential utility of the combination of LC-sense and -antisense libraries for an effective functional validation of genes.

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Copy and paste a formatted citation
Spandidos Publications style
Doh K, Lee Y, Han K, Uhm S, Kim J, Bae Y, Park J, Moon I and Park J: Fabrication of a microarray using a combination of the large circular sense and antisense DNA. Int J Mol Med 25: 113-120, 2010.
APA
Doh, K., Lee, Y., Han, K., Uhm, S., Kim, J., Bae, Y. ... Park, J. (2010). Fabrication of a microarray using a combination of the large circular sense and antisense DNA. International Journal of Molecular Medicine, 25, 113-120. https://doi.org/10.3892/ijmm_00000320
MLA
Doh, K., Lee, Y., Han, K., Uhm, S., Kim, J., Bae, Y., Park, J., Moon, I., Park, J."Fabrication of a microarray using a combination of the large circular sense and antisense DNA". International Journal of Molecular Medicine 25.1 (2010): 113-120.
Chicago
Doh, K., Lee, Y., Han, K., Uhm, S., Kim, J., Bae, Y., Park, J., Moon, I., Park, J."Fabrication of a microarray using a combination of the large circular sense and antisense DNA". International Journal of Molecular Medicine 25, no. 1 (2010): 113-120. https://doi.org/10.3892/ijmm_00000320
Copy and paste a formatted citation
x
Spandidos Publications style
Doh K, Lee Y, Han K, Uhm S, Kim J, Bae Y, Park J, Moon I and Park J: Fabrication of a microarray using a combination of the large circular sense and antisense DNA. Int J Mol Med 25: 113-120, 2010.
APA
Doh, K., Lee, Y., Han, K., Uhm, S., Kim, J., Bae, Y. ... Park, J. (2010). Fabrication of a microarray using a combination of the large circular sense and antisense DNA. International Journal of Molecular Medicine, 25, 113-120. https://doi.org/10.3892/ijmm_00000320
MLA
Doh, K., Lee, Y., Han, K., Uhm, S., Kim, J., Bae, Y., Park, J., Moon, I., Park, J."Fabrication of a microarray using a combination of the large circular sense and antisense DNA". International Journal of Molecular Medicine 25.1 (2010): 113-120.
Chicago
Doh, K., Lee, Y., Han, K., Uhm, S., Kim, J., Bae, Y., Park, J., Moon, I., Park, J."Fabrication of a microarray using a combination of the large circular sense and antisense DNA". International Journal of Molecular Medicine 25, no. 1 (2010): 113-120. https://doi.org/10.3892/ijmm_00000320
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