Chamomile: An anti-inflammatory agent inhibits inducible nitric oxide synthase expression by blocking RelA/p65 activity

  • Authors: Natarajan Bhaskaran, Sanjeev Shukla, Janmejai K. Srivastava, Sanjay Gupta
  • View Affiliations

  • Published online on: Wednesday, December 1, 2010
  • Pages: 935-940
  • DOI: 10.3892/ijmm_00000545

Abstract

Chamomile has long been used in traditional medicine for the treatment of inflammation-related disorders. In this study we investigated the inhibitory effects of chamomile on nitric oxide (NO) production and inducible nitric oxide synthase (iNOS) expression, and explored its potential anti-inflammatory mechanisms using RAW 264.7 macrophages. Chamomile treatment inhibited LPS-induced NO production and significantly blocked IL-1β, IL-6 and TNFα-induced NO levels in RAW 264.7 macrophages. Chamomile caused reduction in LPS-induced iNOS mRNA and protein expression. In RAW 264.7 macrophages, LPS-induced DNA binding activity of RelA/p65 was significantly inhibited by chamomile, an effect that was mediated through the inhibition of IKKβ, the upstream kinase regulating NF-κB/Rel activity, and degradation of inhibitory factor-κB. These results demonstrate that chamomile inhibits NO production and iNOS gene expression by inhibiting RelA/p65 activation and supports the utilization of chamomile as an effective anti-inflammatory agent.
Journal Cover

December 2010
Volume 26 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

2012 Impact Factor: 1.88
Ranked #27/122 Medicine Research and Experimental
(total number of cites)

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APA
Bhaskaran, N., Shukla, S., Srivastava, J., & Gupta, S. (2010). Chamomile: An anti-inflammatory agent inhibits inducible nitric oxide synthase expression by blocking RelA/p65 activity . International Journal of Molecular Medicine, 26(6), 935-940.
MLA
Bhaskaran, Shukla, Srivastava, and Sanjay Gupta. "Chamomile: An anti-inflammatory agent inhibits inducible nitric oxide synthase expression by blocking RelA/p65 activity ." International Journal of Molecular Medicine International Journal of Molecular Medicine 26.6 (2010): 935-940.
Chicago
Bhaskaran, Shukla, Srivastava, and Sanjay Gupta. "Chamomile: An anti-inflammatory agent inhibits inducible nitric oxide synthase expression by blocking RelA/p65 activity ." International Journal of Molecular Medicine International Journal of Molecular Medicine 26 no. 6 (2010): 935-940.