Protective effects of N-acetylcysteine against cadmium-induced damage in cultured rat normal liver cells

  • Authors:
    • Caroline O. Odewumi
    • Veera L.D Badisa
    • Uyen T. Le
    • Lekan M. Latinwo
    • Christopher O. Ikediobi
    • Ramesh B. Badisa
    • Selina F. Darling-Reed
  • Corresponding author:
  • View Affiliations

  • Published online on: Wednesday, December 1, 2010
  • Pages: 243-248
  • DOI: 10.3892/ijmm.2010.564

Abstract

In this study, the protective effects of N-acetylcysteine (NAC), a precursor of reduced glutathione, were studied by measuring the viability, the levels of antioxidant enzymes, and by analyzing the cell cycle in cadmium (Cd)-treated rat liver cells. The cells were treated with 150 µM CdCl2 alone or co-treated with 150 µM CdCl2 and 5 mM NAC (2 h pre-, simultaneous or 2 h post-treatment) for 24 h. The viability of the cells treated with 150 µM CdCl2 alone decreased to 40.1%, while that of the cells co-treated with 5 mM NAC (pre-, simultaneous and post-treatment) significantly increased to 83.7, 86.2 and 83.7%, respectively in comparison to the control cells (100%). The catalase enzyme level decreased to undetectable level in the cells treated with CdCl2 alone, while it significantly increased in the co-treated cells (pre-, simultaneous and post-treatment) to 40.1, 34.3 and 13.2%, respectively. In the cells treated with CdCl2 alone, the glutathione peroxidase enzyme level decreased to 78.3%, while it increased in the co-treated cells (pre-, simultaneous, and post-treatment) to 84.5, 83.3 and 87.9%, respectively. The glutathione reductase enzyme level decreased to 56.1% in the cells treated with cadmium alone, but significantly increased in the cells co treated with NAC (pre-, simultaneous and post-treatment) to 79.5, 78.5 and 78.2%, respectively. Cd caused cell cycle arrest at the S and G2/M phases. The co-treatment with NAC inhibited cell cycle arrest by shifting the cells to the G1 phase. These results clearly show the protective effects of NAC against Cd-induced damage in rat liver cells.
Journal Cover

February 2011
Volume 27 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

2013 Impact Factor: 1.88
Ranked #27/122 Medicine Research and Experimental
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APA
Odewumi, C.O., Badisa, V.L., Le, U.T., Latinwo, L.M., Ikediobi, C.O., Badisa, R.B., & Darling-Reed, S.F. (2011). Protective effects of N-acetylcysteine against cadmium-induced damage in cultured rat normal liver cells. International Journal of Molecular Medicine, 27, 243-248. http://dx.doi.org/10.3892/ijmm.2010.564
MLA
Odewumi, C. O., Badisa, V. L., Le, U. T., Latinwo, L. M., Ikediobi, C. O., Badisa, R. B., Darling-Reed, S. F."Protective effects of N-acetylcysteine against cadmium-induced damage in cultured rat normal liver cells". International Journal of Molecular Medicine 27.2 (2011): 243-248.
Chicago
Odewumi, C. O., Badisa, V. L., Le, U. T., Latinwo, L. M., Ikediobi, C. O., Badisa, R. B., Darling-Reed, S. F."Protective effects of N-acetylcysteine against cadmium-induced damage in cultured rat normal liver cells". International Journal of Molecular Medicine 27, no. 2 (2011): 243-248. http://dx.doi.org/10.3892/ijmm.2010.564