Superior mineralization and neovascularization capacity of adult human metaphyseal periosteum-derived cells for skeletal tissue engineering applications

  • Authors: Daoyun Chen, Hao Shen, Junjie Shao, Yao Jiang, Jianxi Lu, Yaohua He, Chenglong Huang
  • View Affiliations

  • Published online on: Wednesday, March 2, 2011
  • Pages: 707-713
  • DOI: 10.3892/ijmm.2011.634

Abstract

Bone tissue engineering is a promising cell-based strategy to treat bone defects. Mesenchymal stem cells from adult human bone marrow (hBMSCs) are a frequently used cellular source for bone tissue generation. However, the low frequency of these stem cells in adult bone marrow and their limited proliferation restrict their clinical utility. An alternative source of MSCs is the periosteum-derived cells, and these cells appear to be easy to harvest and expand ex vivo. We isolated human metaphyseal periosteum-derived cells (hMPCs) and hBMSCs from the same donors and compared their osteogenic capacity both in vitro and in vivo. After osteogenic induction in monolayer cultures, hMPCs resulted in more robust mineralization and expressed higher mRNA levels of BMP-2, osteopontin and osteocalcin than hBMSCs. Eight weeks after implantation of cellular-β-TCP scaffolds in immunodeficient mice, hMPC implantation showed higher neovascularization and higher percentage of mature bone formation than hBMSC implantation. In conclusion, hMPCs represent a promising cellular candidate for bone tissue engineering.
Journal Cover

May 2011
Volume 27 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

2013 Impact Factor: 1.88
Ranked #27/122 Medicine Research and Experimental
(total number of cites)

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APA
Chen, D., Chen, D., Shen, H., Shen, H., Shao, J., Shao, J., Jiang, Y., Jiang, Y., Lu, J., Lu, J., He, Y., He, Y., Huang, C., & Huang, C. (2011). Superior mineralization and neovascularization capacity of adult human metaphyseal periosteum-derived cells for skeletal tissue engineering applications. International Journal of Molecular Medicine, 27(5), 707-713.
MLA
Chen, Chen, Shen, Shen, Shao, Shao, Jiang, Jiang, Lu, Lu, He, He, Huang, and Chenglong Huang. "Superior mineralization and neovascularization capacity of adult human metaphyseal periosteum-derived cells for skeletal tissue engineering applications." International Journal of Molecular Medicine International Journal of Molecular Medicine 27.5 (2011): 707-713.
Chicago
Chen, Chen, Shen, Shen, Shao, Shao, Jiang, Jiang, Lu, Lu, He, He, Huang, and Chenglong Huang. "Superior mineralization and neovascularization capacity of adult human metaphyseal periosteum-derived cells for skeletal tissue engineering applications." International Journal of Molecular Medicine International Journal of Molecular Medicine 27 no. 5 (2011): 707-713.