Dynamin-related protein 1 is implicated in endoplasmic reticulum stress-induced pancreatic β-cell apoptosis

  • Authors: Liang Peng, Xiuli Men, Wenjian Zhang, Haiyan Wang, Shiqing  Xu, Mei Xu, Yaping Xu, Wenying Yang, Jinning Lou
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  • Published online on: Thursday, April 28, 2011
  • Pages: 161-169
  • DOI: 10.3892/ijmm.2011.684

Abstract

Pancreatic β-cell dysfunction is a critical component in the pathogenesis of diabetes. Endoplasmic reticulum (ER) stress is one of the factors that induces pancreatic β-cell dysfunction, but the underlying mechanisms have not been well elucidated. In this study, we report that a mitochondrial fission modulator, dynamin-related protein 1 (DRP-1), plays an important role in ER stress-induced β-cell apoptosis. Induction of DRP-1 expression significantly promoted ER stress-induced apoptosis in the DRP-1 WT (DRP-1 wild- type) inducible β-cell line, but not in the DRP-1 K38A (a dominant negative mutant of DRP-1) inducible β-cell line. We further demonstrated that the mitochondrial membrane potential decreased, and that cytochrome c release, caspase-3 activation and generation of reactive oxygen species (ROS) were enhanced by induction of DRP-1 WT, but prevented by DRP-1 K38A in pancreatic β-cells under ER stress conditions. These results indicate that DRP-1 mediates ER stress-induced pancreatic β-cell apoptosis.
Journal Cover

August 2011
Volume 28 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

2013 Impact Factor: 1.88
Ranked #27/122 Medicine Research and Experimental
(total number of cites)

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APA
Peng, L., Men, X., Zhang, W., Wang, H., Xu, S., Xu, M., Xu, Y., Yang, W., & Lou, J. (2011). Dynamin-related protein 1 is implicated in endoplasmic reticulum stress-induced pancreatic β-cell apoptosis. International Journal of Molecular Medicine, 28(2), 161-169.
MLA
Peng, Men, Zhang, Wang, Xu, Xu, Xu, Yang, and Jinning Lou. "Dynamin-related protein 1 is implicated in endoplasmic reticulum stress-induced pancreatic β-cell apoptosis." International Journal of Molecular Medicine International Journal of Molecular Medicine 28.2 (2011): 161-169.
Chicago
Peng, Men, Zhang, Wang, Xu, Xu, Xu, Yang, and Jinning Lou. "Dynamin-related protein 1 is implicated in endoplasmic reticulum stress-induced pancreatic β-cell apoptosis." International Journal of Molecular Medicine International Journal of Molecular Medicine 28 no. 2 (2011): 161-169.