Selective cell cycle arrest and induction of apoptosis in human prostate cancer cells by a polyphenol-rich extract of Solanum nigrum

  • Authors:
    • Akbar Nawab
    • Vijay S. Thakur
    • Mohammad Yunus
    • Abbas Ali Mahdi
    • Sanjay Gupta
  • View Affiliations

  • Published online on: November 10, 2011     https://doi.org/10.3892/ijmm.2011.835
  • Pages: 277-284
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Abstract

Progression of prostate cancer is associated with escape of tumor cells from cell cycle arrest and apoptosis. Agents capable of selectively eliminating cancer cells by cell cycle arrest and/or induction of apoptosis offer a highly desirable approach. Here we demonstrate that a polyphenolic extract derived from ripe berries of Solanum nigrum (SN) differentially causes cell cycle arrest and apoptosis in various human prostate cancer cells without affecting normal prostate epithelial cells. Virally transformed normal human prostate epithelial PZ-HPV-7 cells and their cancer counterpart CA-HPV-10 cells, were used to evaluate the growth-inhibitory effects of the SN extract. SN treatment (5-20 µg/ml) of PZ-HPV-7 cells resulted in growth inhibitory responses of low magnitude. In sharp contrast, SN treatment of CA-HPV-10 cells increased cytotoxicity, decreased cell viability and induced apoptosis. Similar results were noted in the human prostate cancer LNCaP, 22Rv1, DU145 and PC-3 cell lines, where significant reductions in cell viability and induction of apoptosis was observed in all these cells, an effect independent of disease stage and androgen association. Cell cycle analysis revealed that SN treatment (5-20 µg/ml) resulted in a dose-dependent G2/M phase arrest and subG1 accumulation in the CA-HPV-10 but not in the PZ-HPV-7 cell line. Our results, for the first time, demonstrate that the SN extract is capable of selectively inhibiting cellular proliferation and accelerating apoptotic events in prostate cancer cells. SN may be developed as a promising therapeutic and/or preventive agent against prostate cancer.

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February 2012
Volume 29 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Nawab A, Thakur VS, Yunus M, Ali Mahdi A and Gupta S: Selective cell cycle arrest and induction of apoptosis in human prostate cancer cells by a polyphenol-rich extract of Solanum nigrum. Int J Mol Med 29: 277-284, 2012.
APA
Nawab, A., Thakur, V.S., Yunus, M., Ali Mahdi, A., & Gupta, S. (2012). Selective cell cycle arrest and induction of apoptosis in human prostate cancer cells by a polyphenol-rich extract of Solanum nigrum. International Journal of Molecular Medicine, 29, 277-284. https://doi.org/10.3892/ijmm.2011.835
MLA
Nawab, A., Thakur, V. S., Yunus, M., Ali Mahdi, A., Gupta, S."Selective cell cycle arrest and induction of apoptosis in human prostate cancer cells by a polyphenol-rich extract of Solanum nigrum". International Journal of Molecular Medicine 29.2 (2012): 277-284.
Chicago
Nawab, A., Thakur, V. S., Yunus, M., Ali Mahdi, A., Gupta, S."Selective cell cycle arrest and induction of apoptosis in human prostate cancer cells by a polyphenol-rich extract of Solanum nigrum". International Journal of Molecular Medicine 29, no. 2 (2012): 277-284. https://doi.org/10.3892/ijmm.2011.835