microRNA-29a suppresses cell proliferation by targeting SPARC in hepatocellular carcinoma

  • Authors:
    • Xu-Chao Zhu
    • Qiong-Zhu Dong
    • Xiao-Fei Zhang
    • Biao Deng
    • Hu-Liang Jia
    • Qin-Hai Ye
    • Lun-Xiu Qin
    • Xing-Zhong Wu
  • Corresponding author:
  • View Affiliations

  • Published online on: Tuesday, September 25, 2012
  • Pages: 1321-1326
  • DOI: 10.3892/ijmm.2012.1140

Abstract

In the present study, we constructed a lentivirus vector encoding the miR-29a precursor and established two stably infected cell lines, PLC-29a and 97L-29a. The overexpression of miR-29a was confirmed by TaqMan RT-PCR and significantly suppressed the growth of the hepatocellular carcinoma cell lines MHCC-97L and PLC. Dual-luciferase reporter assays indicated that the SPARC mRNA 3'UTR was directly targeted by miR-29a since the mutated 3'UTR was not affected. Silencing SPARC expression by RNAi knockdown resulted in a similar effect as miR-29a overexpression on hepatocellular carcinoma (HCC) cell growth regulation. Anti-miR-29a oligonucleotides (AMOs) upregulated the levels of SPARC in the HCC cells. The phosphorylation of AKT/mTOR downstream of SPARC was inhibited in miR-29a-overexpressing HCC cells. We further examined and compared the expression levels of miR-29a in HCC tissues and the corresponding nearby non-cancerous liver tissues of 110 patients with HCC by qRT-PCR, and significantly lower expression of miR-29a was observed in the tissues affected by HCC. Our findings demonstrate that the expression of miR-29a is important in the regulation of the SPARC-AKT pathway and HCC growth.
Journal Cover

December 2012
Volume 30 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

2013 Impact Factor: 1.88
Ranked #27/122 Medicine Research and Experimental
(total number of cites)

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APA
Zhu, X., Dong, Q., Zhang, X., Deng, B., Jia, H., Ye, Q. ... Wu, X. (2012). microRNA-29a suppresses cell proliferation by targeting SPARC in hepatocellular carcinoma. International Journal of Molecular Medicine, 30, 1321-1326. http://dx.doi.org/10.3892/ijmm.2012.1140
MLA
Zhu, X., Dong, Q., Zhang, X., Deng, B., Jia, H., Ye, Q., Qin, L., Wu, X."microRNA-29a suppresses cell proliferation by targeting SPARC in hepatocellular carcinoma". International Journal of Molecular Medicine 30.6 (2012): 1321-1326.
Chicago
Zhu, X., Dong, Q., Zhang, X., Deng, B., Jia, H., Ye, Q., Qin, L., Wu, X."microRNA-29a suppresses cell proliferation by targeting SPARC in hepatocellular carcinoma". International Journal of Molecular Medicine 30, no. 6 (2012): 1321-1326. http://dx.doi.org/10.3892/ijmm.2012.1140