A method for the ultrastructural preservation of tiny percutaneous needle biopsy material from skeletal muscle

  • Authors: Tiziana Pietrangelo, Stefano Perni, Guglielmo Di Tano, Giorgio Fanò-Illic, Clara Franzini-Armstrong
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  • Published online on: Tuesday, July 23, 2013
  • Pages: 965-970
  • DOI: 10.3892/ijmm.2013.1454

Abstract

Skeletal muscle biopsies require transecting the muscle fibers resulting, in structural damage near the cut ends. Classically, the optimal ultrastructural preservation has been obtained by the use of relatively large biopsies in which the tissue fibers are restrained by ligating to a suitable retaining support prior to excision, and by examining regions at some distance from the cut ends. However, these methods require invasive surgical procedures. In the present study, we present and substantiate an alternative approach that allows for the excellent ultrastructural preservation of needle biopsy samples, even the very small samples obtained through tiny percutaneous needle biopsy (TPNB). TPNB represents an advantage, relative to standard muscle biopsy techniques and to other needle biopsies currently in use, as in addition to not requiring a skin incision, it leaves no scars in the muscle and requires an extremely brief recovery period. It is most appropriate for obtaining repeated samples in horizontal studies, e.g., in order to follow changes with athletic training and/or aging in a single individual and for studies of sarcopenic muscles in elderly patients. Due to the small size of the sample, TPNB may present limited usefulness for classical pathology diagnostics. However, it offers the major advantage of allowing multiple samples within a single session and this may be useful under specific circumstances.
Journal Cover

October 2013
Volume 32 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

2012 Impact Factor: 1.957
Ranked #26/121 Medicine Research and Experimental
(total number of cites)

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APA
Pietrangelo, T., Perni, S., Di Tano, G., Fanò-Illic, G., & Franzini-Armstrong, C. (2013). A method for the ultrastructural preservation of tiny percutaneous needle biopsy material from skeletal muscle. International Journal of Molecular Medicine, 32(4), 965-970.
MLA
Pietrangelo, Perni, Di Tano, Fanò-Illic, and Clara Franzini-Armstrong. "A method for the ultrastructural preservation of tiny percutaneous needle biopsy material from skeletal muscle." International Journal of Molecular Medicine International Journal of Molecular Medicine 32.4 (2013): 965-970.
Chicago
Pietrangelo, Perni, Di Tano, Fanò-Illic, and Clara Franzini-Armstrong. "A method for the ultrastructural preservation of tiny percutaneous needle biopsy material from skeletal muscle." International Journal of Molecular Medicine International Journal of Molecular Medicine 32 no. 4 (2013): 965-970.