Protective effects of apigenin against 1-methyl-4-phenylpyridinium ion‑induced neurotoxicity in PC12 cells

  • Authors:
    • Weihai Liu
    • Songzhi Kong
    • Qingfeng Xie
    • Jiyan Su
    • Wenjie Li
    • Huizhen Guo
    • Shanshan Li
    • Xuexuan Feng
    • Ziren Su
    • Yang Xu
    • Xiaoping Lai
  • View Affiliations

  • Published online on: December 30, 2014     https://doi.org/10.3892/ijmm.2014.2056
  • Pages: 739-746
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Abstract

Parkinson's disease is recognized as the second most common neurodegenerative disorder after Alzheimer's disease, characterized by the loss of dopominergic neurons in the substantia nigra pars compacta and can be experimentally mimicked by the use of the neurotoxin, 1‑methyl‑4‑phenylpyridinium ion (MPP+), in in vitro models. In this study, we investigated the potential protective effects of apigenin (AP), galangin and genkwanin, naturally occurring plant flavonoids, on the MPP+‑induced cytotoxicity in cultured rat adrenal pheochromocytoma cells (PC12 cells). The PC12 cells were pre-treated with various concentrations of the test compounds for 4 h, followed by the challenge with 1,000 µM MPP+ for 48 h. We found that only pre-treatment with AP (3, 6 and 12 µM) before injury significantly increased cell viability, decreased the release of lactate dehydrogenase, reduced the level of intracellular reactive oxygen species and elevated mitochondrial membrane potential in the MPP+‑treated PC12 cells. In addition, AP markedly suppressed the increased rate of apoptosis and the reduced Bcl‑2/Bax ratio induced by MPP+ in the PC12 cells. Taken together, the findings of this study demonstrate that AP exerts neuroprotective effects against MPP+‑induced neurotoxicity in PC12 cells, at least in part, through the inhibition of oxidative damage and the suppression of apoptosis through the mitochondrial pathway.
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March-2015
Volume 35 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Liu W, Kong S, Xie Q, Su J, Li W, Guo H, Li S, Feng X, Su Z, Xu Y, Xu Y, et al: Protective effects of apigenin against 1-methyl-4-phenylpyridinium ion‑induced neurotoxicity in PC12 cells. Int J Mol Med 35: 739-746, 2015.
APA
Liu, W., Kong, S., Xie, Q., Su, J., Li, W., Guo, H. ... Lai, X. (2015). Protective effects of apigenin against 1-methyl-4-phenylpyridinium ion‑induced neurotoxicity in PC12 cells. International Journal of Molecular Medicine, 35, 739-746. https://doi.org/10.3892/ijmm.2014.2056
MLA
Liu, W., Kong, S., Xie, Q., Su, J., Li, W., Guo, H., Li, S., Feng, X., Su, Z., Xu, Y., Lai, X."Protective effects of apigenin against 1-methyl-4-phenylpyridinium ion‑induced neurotoxicity in PC12 cells". International Journal of Molecular Medicine 35.3 (2015): 739-746.
Chicago
Liu, W., Kong, S., Xie, Q., Su, J., Li, W., Guo, H., Li, S., Feng, X., Su, Z., Xu, Y., Lai, X."Protective effects of apigenin against 1-methyl-4-phenylpyridinium ion‑induced neurotoxicity in PC12 cells". International Journal of Molecular Medicine 35, no. 3 (2015): 739-746. https://doi.org/10.3892/ijmm.2014.2056