Berberine regulates melanin synthesis by activating PI3K/AKT, ERK and GSK3β in B16F10 melanoma cells

  • Authors:
    • Young Chan Song
    • Yonghee Lee
    • Hyeong Mi Kim
    • Moo Yeol Hyun
    • Yun Young Lim
    • Kye Yong Song
    • Beom Joon Kim
  • View Affiliations

  • Published online on: February 26, 2015     https://doi.org/10.3892/ijmm.2015.2113
  • Pages: 1011-1016
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Abstract

Berberine, an isoquinoline alkaloid, has a wide range of beneficial properties, including anti‑bacterial, anti‑inflammatory, anti‑cancer, and cholesterol‑lowering effects. Recently findings suggest that berberine improves glucose and lipid metabolism disorders. In the present study, we examined the mechanism underlying the inhibitory effect of berberine on α‑melanocyte‑stimulating hormone (α‑MSH)‑stimulated B16F10 melanoma cells. The results showed that berberine attenuated α‑MSH induction of the microphthalmia‑associated transcription factor (MITF) and tyrosinase in a dose‑dependent manner. To elucidate the mechanism underlying the inhibitory effect of berberine, we examined the effect of α‑MSH‑stimulated phosphorylation of PI3K/AKT, ERK, and GSK3β. The results showed that treatment with berberine resulted in a reduction in the phosphorylation of PI3K/AKT, ERK, and GSK3β. Taken together, the results suggested that berberine inhibits melanin synthesis and tyrosinase activity by downregulating the expression of MITF and tyrosinase. Thus, these findings may contribute to the potential application of berberine in the prevention and treatment of skin pigmentation disorders.
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April-2015
Volume 35 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Song YC, Lee Y, Kim HM, Hyun MY, Lim YY, Song KY and Kim BJ: Berberine regulates melanin synthesis by activating PI3K/AKT, ERK and GSK3β in B16F10 melanoma cells. Int J Mol Med 35: 1011-1016, 2015.
APA
Song, Y.C., Lee, Y., Kim, H.M., Hyun, M.Y., Lim, Y.Y., Song, K.Y., & Kim, B.J. (2015). Berberine regulates melanin synthesis by activating PI3K/AKT, ERK and GSK3β in B16F10 melanoma cells. International Journal of Molecular Medicine, 35, 1011-1016. https://doi.org/10.3892/ijmm.2015.2113
MLA
Song, Y. C., Lee, Y., Kim, H. M., Hyun, M. Y., Lim, Y. Y., Song, K. Y., Kim, B. J."Berberine regulates melanin synthesis by activating PI3K/AKT, ERK and GSK3β in B16F10 melanoma cells". International Journal of Molecular Medicine 35.4 (2015): 1011-1016.
Chicago
Song, Y. C., Lee, Y., Kim, H. M., Hyun, M. Y., Lim, Y. Y., Song, K. Y., Kim, B. J."Berberine regulates melanin synthesis by activating PI3K/AKT, ERK and GSK3β in B16F10 melanoma cells". International Journal of Molecular Medicine 35, no. 4 (2015): 1011-1016. https://doi.org/10.3892/ijmm.2015.2113