Cellular response of chondrocytes to magnesium alloys for orthopedic applications

Liao,Yi; Xu,Qingli; Zhang,Jian; Niu,Jialing; Yuan,Guangyin; Jiang,Yao; He,Yaohua; Wang,Xinling

doi:10.3892/ijmm.2015.2211

Cellular response of chondrocytes to magnesium alloys for orthopedic applications

Authors:
- Yi Liao
- Qingli Xu
- Jian Zhang
- Jialing Niu
- Guangyin Yuan
- Yao Jiang
- Yaohua He
- Xinling Wang
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Affiliations: Department of Orthopaedics, The Fifth People's Hospital of Shanghai, Fudan University, Shanghai 200240, P.R. China, Department of Orthopaedics, The Huashan Hospital Baoshan Branch, Fudan University, Shanghai 200431, P.R. China, National Engineering Research Center of Light Alloys Net Forming (LAF), School of Materials Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, P.R. China, Department of Orthopaedics, The Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai 200233, P.R. China, Department of Radiology, The Fifth People's Hospital of Shanghai, Fudan University, Shanghai 200240, P.R. China
Published online on: May 14, 2015 https://doi.org/10.3892/ijmm.2015.2211
Pages: 73-82
Copyright: © Liao et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

In the present study, the effects of Mg-Nd-Zn-Zr (JDBM), brushite (CaHPO4·2H2O)-coated JDBM (C-JDBM), AZ31, WE43, pure magnesium (Mg) and Ti alloy (TC4) on rabbit chondrocytes were investigated in vitro. Adhesion experiments revealed the satisfactory morphology of chondrocytes on the surface of all samples. An indirect cytotoxicity test using MTT assay revealed that C‑JDBM and TC4 exhibited results similar to those of the negative control, better than those obtained with JDBM, AZ31, WE43 and pure Mg (p<0.05). There were no statistically significant differences observed between the JDBM, AZ31, WE43 and pure Mg group (p>0.05). The results of indirect cell cytotoxicity and proliferation assays, as well as those of apoptosis assay, glycosaminoglycan (GAG) quantification, assessment of collagen Ⅱ (Col Ⅱ) levels and RT-qPCR revealed a similar a trend as was observed with MTT assay. These findings suggested that the JDBM alloy was highly biocompatible with chondrocytes in vitro, yielding results similar to those of AZ31, WE43 and pure Mg. Furthermore, CaHPO4·2H2O coating significantly improved the biocompatibility of this alloy.

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1	Song G, Atrens A, St John D, Wu X and Nairn J: The anodic dissolution of magnesium in chloride and sulphate solutions. Corros Sci. 39:1981–2004. 1997. View Article : Google Scholar
2	Witte F, Kaese V, Haferkamp H, Switzer E, Meyer-Lindenberg A, Wirth CJ and Windhagen H: In vivo corrosion of four magnesium alloys and the associated bone response. Biomaterials. 26:3557–3563. 2005. View Article : Google Scholar
3	Witte F, Fischer J, Nellesen J, Crostack HA, Kaese V, Pisch A, Beckmann F and Windhagen H: In vitro and in vivo corrosion measurements of magnesium alloys. Biomaterials. 27:1013–1018. 2006. View Article : Google Scholar
4	Xu L, Yu G, Zhang E, Pan F and Yang K: In vivo corrosion behavior of Mg-Mn-Zn alloy for bone implant application. J Biomed Mater Res A. 83:703–711. 2007. View Article : Google Scholar : PubMed/NCBI
5	Kannan MB and Raman RKS: In vitro degradation and mechanical integrity of calcium-containing magnesium alloys in modified-simulated body fluid. Biomaterials. 29:2306–2314. 2008. View Article : Google Scholar : PubMed/NCBI
6	Staiger MP, Pietak AM, Huadmai J and Dias G: Magnesium and its alloys as orthopedic biomaterials: A review. Biomaterials. 27:1728–1734. 2006. View Article : Google Scholar
7	Xu L, Pan F, Yu G, Yang L, Zhang E and Yang K: In vitro and in vivo evaluation of the surface bioactivity of a calcium phosphate coated magnesium alloy. Biomaterials. 30:1512–1523. 2009. View Article : Google Scholar
8	Witte F, Hort N, Vogt C, Cohen S, Kainer KU, Willumeit R and Feyerabend F: Degradable biomaterials based on magnesium corrosion. Curr Opin Solid State Mater Sci. 12:63–72. 2008. View Article : Google Scholar
9	El-Rahman SS: Neuropathology of aluminum toxicity in rats (glutamate and GABA impairment). Pharmacol Res. 47:189–194. 2003. View Article : Google Scholar : PubMed/NCBI
10	Hirano S and Suzuki KT: Exposure, metabolism, and toxicity of rare earths and related compounds. Environ Health Perspect. 104(Suppl 1): S85–S95. 1996. View Article : Google Scholar
11	Yuan G, Zhang X, Niu J, Tao H, Chen D, He Y, Jiang Y and Ding W: Research progress of new type of degradable biomedical magnesium alloys JDBM. Chin J Nonferrous Met. 21:2476–2488. 2011.
12	Liao Y, Ouyang Y, Niu J, Zhang J, Wang Y, Zhu Z, Yuan G, He Y and Jiang Y: In vitro response of chondrocytes to a biodegradable Mg-Nd-Zn-Zr alloy. Mater Lett. 83:206–208. 2012. View Article : Google Scholar
13	Niu J, Yuan G, Liao Y, Mao L, Zhang J, Wang Y, Huang F, Jiang Y, He Y and Ding W: Enhanced biocorrosion resistance and biocompatibility of degradable Mg-Nd-Zn-Zr alloy by brushite coating. Mater Sci Eng C Mater Biol Appl. 33:4833–4841. 2013. View Article : Google Scholar : PubMed/NCBI
14	Lin YJ, Yen CN, Hu YC, Wu YC, Liao CJ and Chu IM: Chondrocytes culture in three-dimensional porous alginate scaffolds enhanced cell proliferation, matrix synthesis and gene expression. J Biomed Mater Res A. 88:23–33. 2009. View Article : Google Scholar
15	Zhang XB, Yuan GY, Mao L, Niu JL and Ding WJ: Biocorrosion properties of as-extruded Mg-Nd-Zn-Zr alloy compared with commercial AZ31 and WE43 alloys. Mater Lett. 66:209–211. 2012. View Article : Google Scholar
16	Feyerabend F, Fischer J, Holtz J, Witte F, Willumeit R, Drücker H, Vogt C and Hort N: Evaluation of short-term effects of rare earth and other elements used in magnesium alloys on primary cells and cell lines. Acta Biomater. 6:1834–1842. 2010. View Article : Google Scholar
17	Geng F, Tan LL, Jin XX, Yang JY and Yang K: The preparation, cytocompatibility, and in vitro biodegradation study of pure β-TCP on magnesium. J Mater Sci Mater Med. 20:1149–1157. 2009. View Article : Google Scholar : PubMed/NCBI
18	Popat KC, Leoni L, Grimes CA and Desai TA: Influence of engineered titania nanotubular surfaces on bone cells. Biomaterials. 28:3188–3197. 2007. View Article : Google Scholar : PubMed/NCBI
19	Keim S, Brunner JG, Fabry B and Virtanen S: Control of magnesium corrosion and biocompatibility with biomimetic coatings. J Biomed Mater Res B Appl Biomater. 96:84–90. 2011. View Article : Google Scholar
20	Li J, Song Y, Zhang S, Zhao C, Zhang F, Zhang X, Cao L, Fan Q and Tang T: In vitro responses of human bone marrow stromal cells to a fluoridated hydroxyapatite coated biodegradable Mg-Zn alloy. Biomaterials. 31:5782–5788. 2010. View Article : Google Scholar : PubMed/NCBI
21	Zhang SX, Li JA, Song Y, Zhao C, Zhang X, Xie C, Zhang Y, Tao H, He Y, Jiang Y and Bian YJ: In vitro degradation, hemolysis and MC3T3-E1 cell adhesion of biodegradable Mg-Zn alloy. Mater Sci Eng C Mater Biol Appl. 29:1907–1912. 2009. View Article : Google Scholar
22	Witte F, Feyerabend F, Maier P, Fischer J, Störmer M, Blawert C, Dietzel W and Hort N: Biodegradable magnesium-hydroxy-apatite metal matrix composites. Biomaterials. 28:2163–2174. 2007. View Article : Google Scholar : PubMed/NCBI
23	Serre CM, Papillard M, Chavassieux P, Voegel JC and Boivin G: Influence of magnesium substitution on a collagen-apatite biomaterial on the production of a calcifying matrix by human osteoblasts. J Biomed Mater Res. 42:626–633. 1998. View Article : Google Scholar : PubMed/NCBI
24	Yang C, Yuan G, Zhang J, Tang Z, Zhang X and Dai K: Effects of magnesium alloys extracts on adult human bone marrow-derived stromal cell viability and osteogenic differentiation. Biomed Mater. 5:0450052010. View Article : Google Scholar : PubMed/NCBI
25	Nair S, Sasidharan A, Divya Rani VV, Menon D, Nair S, Manzoor K and Raina S: Role of size scale of ZnO nanoparticles and microparticles on toxicity toward bacteria and osteoblast cancer cells. J Mater Sci Mater Med. 20(Suppl 1): S235–S241. 2009. View Article : Google Scholar
26	Chaipinyo K, Oakes BW and van Damme MPI: Effects of growth factors on cell proliferation and matrix synthesis of low-density, primary bovine chondrocytes cultured in collagen I gels. J Orthop Res. 20:1070–1078. 2002. View Article : Google Scholar : PubMed/NCBI
27	Brodkin KR, García AJ and Levenston ME: Chondrocyte phenotypes on different extracellular matrix monolayers. Biomaterials. 25:5929–5938. 2004. View Article : Google Scholar : PubMed/NCBI
28	Martin I, Suetterlin R, Baschong W, Heberer M, Vunjak-Novakovic G and Freed LE: Enhanced cartilage tissue engineering by sequential exposure of chondrocytes to FGF-2 during 2D expansion and BMP-2 during 3D cultivation. J Cell Biochem. 83:121–128. 2001. View Article : Google Scholar : PubMed/NCBI
29	Gagne TA, Chappell-Afonso K, Johnson JL, McPherson JM, Oldham CA, Tubo RA, Vaccaro C and Vasios GW: Enhanced proliferation and differentiation of human articular chondrocytes when seeded at low cell densities in alginate in vitro. J Orthop Res. 18:882–890. 2000. View Article : Google Scholar
30	Du H, Wei Z, Wang H, Zhang E, Zuo L and Du L: Surface microstructure and cell compatibility of calcium silicate and calcium phosphate composite coatings on Mg-Zn-Mn-Ca alloys for biomedical application. Colloids Surf B Biointerfaces. 83:96–102. 2011. View Article : Google Scholar
31	Hench LL: Bioceramics. J Am Ceram Soc. 81:1705–1728. 1998. View Article : Google Scholar
32	Song GL and Song SZ: A possible biodegradable magnesium implant material. Adv Eng Mater. 9:298–302. 2007. View Article : Google Scholar
33	Xu L, Zhang E and Yang K: Phosphating treatment and corrosion properties of Mg-Mn-Zn alloy for biomedical application. J Mater Sci Mater Med. 20:859–867. 2009. View Article : Google Scholar
34	Rude RK, Gruber HE, Wei LY, Frausto A and Mills BG: Magnesium deficiency: Effect on bone and mineral metabolism in the mouse. Calcif Tissue Int. 72:32–41. 2003. View Article : Google Scholar
35	Paul W and Sharma CP: Nanoceramic matrices: Biomedical applications. Am J Biochem Biotechnol. 2:41–48. 2006. View Article : Google Scholar
36	Janning C, Willbold E, Vogt C, Nellesen J, Meyer-Lindenberg A, Windhagen H, Thorey F and Witte F: Magnesium hydroxide temporarily enhancing osteoblast activity and decreasing the osteoclast number in peri-implant bone remodelling. Acta Biomater. 6:1861–1868. 2010. View Article : Google Scholar
37	Ilich JZ and Kerstetter JE: Nutrition in bone health revisited: A story beyond calcium. J Am Coll Nutr. 19:715–737. 2000. View Article : Google Scholar
38	Feng B, Weng J, Yang BC, Qu SX and Zhang XD: Characterization of titanium surfaces with calcium and phosphate and osteoblast adhesion. Biomaterials. 25:3421–3428. 2004. View Article : Google Scholar : PubMed/NCBI
39	TenHuisen KS and Brown PW: Effects of magnesium on the formation of calcium-deficient hydroxyapatite from CaHPO ₄·2H₂O and Ca₄(PO₄)₂O. J Biomed Mater Res. 36:306–314. 1997. View Article : Google Scholar : PubMed/NCBI
40	Witte F, Reifenrath J, Müller PP, Crostack H-A, Nellesen J, Bach FW, Bormann D and Rudert M: Cartilage repair on magnesium scaffolds used as a subchondral bone replacement. Materialwiss Werkstofftech. 37:504–508. 2006. View Article : Google Scholar