Ginsenoside Rg1 reduces aldosterone-induced autophagy via the AMPK/mTOR pathway in NRK-52E cells

  • Authors:
    • Li Wang
    • Nan Mao
    • Rui‑Zhi Tan
    • Hong‑Lian Wang
    • Ji Wen
    • Yu‑Hang Liu
    • Md Furhad
    • Jun‑Ming Fan
  • View Affiliations

  • Published online on: June 10, 2015     https://doi.org/10.3892/ijmm.2015.2242
  • Pages: 518-526
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Abstract

Aldosterone is a steroid hormone secreted from the adrenal cortex, which regulates blood pressure. Higher concentrations of aldosterone can cause several diseases, including hypertension, diabetic nephropathy and chronic kidney disease. Previous reports have demonstrated that aldosterone has a pathogenic role in renal injury via reactive oxygen species (ROS), which involves the regulation of autophagy. However, whether aldosterone can induce autophagy in renal tubular cells remains to be elucidated. In the present study, elevated autophagy was observed in rat renal tubular NRK‑52E cells exposed to aldosterone, which was demonstrated by the increased number of autophagosomes, conversion of LC3‑I to LC3‑II and the expression of Beclin‑1. The enhanced autophagy was accompanied by increased production of intracellular ROS, which was reversed by N‑acetylcysteine, a specific inhibitor of ROS signaling. Furthermore, treatment with ginsenoside Rg1 reduced the aldosterone‑induced autophagy and production of ROS, possibly through reducing the phosphorylation of AMPK and preserving mTOR activity. These findings demonstrated that aldosterone promoted ROS generation and increased autophagy in the NRK‑52E cells. Ginsenoside Rg1 effectively relieved aldosterone‑induced oxidative stress and abnormal autophagy, suggesting that Rg1 may be used as a potential therapeutic drug to inhibit the renal injury, which is induced by aldosterone.
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August-2015
Volume 36 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Wang L, Mao N, Tan RZ, Wang HL, Wen J, Liu YH, Furhad M and Fan JM: Ginsenoside Rg1 reduces aldosterone-induced autophagy via the AMPK/mTOR pathway in NRK-52E cells. Int J Mol Med 36: 518-526, 2015.
APA
Wang, L., Mao, N., Tan, R., Wang, H., Wen, J., Liu, Y. ... Fan, J. (2015). Ginsenoside Rg1 reduces aldosterone-induced autophagy via the AMPK/mTOR pathway in NRK-52E cells. International Journal of Molecular Medicine, 36, 518-526. https://doi.org/10.3892/ijmm.2015.2242
MLA
Wang, L., Mao, N., Tan, R., Wang, H., Wen, J., Liu, Y., Furhad, M., Fan, J."Ginsenoside Rg1 reduces aldosterone-induced autophagy via the AMPK/mTOR pathway in NRK-52E cells". International Journal of Molecular Medicine 36.2 (2015): 518-526.
Chicago
Wang, L., Mao, N., Tan, R., Wang, H., Wen, J., Liu, Y., Furhad, M., Fan, J."Ginsenoside Rg1 reduces aldosterone-induced autophagy via the AMPK/mTOR pathway in NRK-52E cells". International Journal of Molecular Medicine 36, no. 2 (2015): 518-526. https://doi.org/10.3892/ijmm.2015.2242