Suppression of TPA-induced cancer cell invasion by Peucedanum japonicum Thunb. extract through the inhibition of PKCα/NF-κB-dependent MMP-9 expression in MCF-7 cells

Kim,Jeong-Mi; Noh,Eun-Mi; Kim,Ha-Rim; Kim,Mi-Seong; Song,Hyun-Kyung; Lee,Minok; Yang,Sei-Hoon; Lee,Guem-San; Moon,Hyoung-Chul; Kwon,Kang-Beom; Lee,Young-Rae

doi:10.3892/ijmm.2015.2417

Suppression of TPA-induced cancer cell invasion by Peucedanum japonicum Thunb. extract through the inhibition of PKCα/NF-κB-dependent MMP-9 expression in MCF-7 cells

Authors:
- Jeong-Mi Kim
- Eun-Mi Noh
- Ha-Rim Kim
- Mi-Seong Kim
- Hyun-Kyung Song
- Minok Lee
- Sei-Hoon Yang
- Guem-San Lee
- Hyoung-Chul Moon
- Kang-Beom Kwon
- Young-Rae Lee
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Affiliations: Center for Metabolic Function Regulation, Wonkwang University School of Medicine, Iksan, Jeonbuk 570-749, Republic of Korea, Department of Internal Medicine, Wonkwang University School of Medicine, Iksan, Jeonbuk 570-749, Republic of Korea, Department of Herbology, Wonkwang University School of Korean Medicine, Iksan, Jeonbuk 570-749, Republic of Korea, Institute of Customized Physical Therapy, Gwanju Metropolitan City 506-303, Republic of Korea
Published online on: November 19, 2015 https://doi.org/10.3892/ijmm.2015.2417
Pages: 108-114
Copyright: © Kim et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Metastatic cancers spread from their site of origin (the primary site) to other parts of the body. Matrix metalloproteinase-9 (MMP-9), which degrades the extracellular matrix, is important in metastatic cancers as it plays a major role in cancer cell invasion. The present study examined the inhibitory effect of an ethanol extract of Peucedanum japonicum Thunb. (PJT) on MMP-9 expression and the invasion of MCF-7 breast cancer cells induced by 12-O-tetradecanoylphorbol-13-acetate (TPA). Western blot analysis, gelatin zymography, and reverse transcription-quantitative PCR revealed that PJT significantly suppressed MMP-9 expression and activation in a dose-dependent manner. Furthermore, PJT attenuated TPA-induced nuclear translocation and the transcriptional activation of nuclear factor (NF)-κB. The results indicated that the PJT-mediated inhibition of TPA-induced MMP-9 expression and cell invasion involved the suppression of the PKCα/NF-κB pathway in MCF-7 cells. Thus, the inhibition of MMP-9 expression by PJT may have potential value as a therapy for restricting the invasiveness of breast cancer.

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