Quantitation of macrophage migration inhibitory factor (MIF) using the one-step sandwich enzyme immunosorbent assay: elevated serum MIF concentrations in patients with autoimmune diseases and identification of MIF in erythrocytes.

  • Authors: Y Mizue, J Nishihira, T Miyazaki, S Fujiwara, M Chida, K Nakamura, K Kikuchi, M Mukai
  • View Affiliations

  • Published online on: Saturday, April 1, 2000
  • Pages: 397-800
  • DOI: 10.3892/ijmm.5.4.397

Abstract

We raised monoclonal antibodies against human macrophage migration inhibitory factor (MIF), and developed a one-step sandwich enzyme-linked immunosorbent assay (ELISA) method highly specific for human MIF. The ELISA system utilizes a solid phase monoclonal antibody as a capture antibody and a horseradish peroxidase-conjugated monoclonal antibody as a detector antibody. We used this ELISA method to evaluate the serum level of MIF in 240 healthy volunteers (140 males and 100 females). We found no significant difference in MIF concentration with respect to age. A significant difference was found with respect to sex, with the mean value (+/- SD) for male subjects of 5.3+/-2.3, and that for female subjects of 4.6+/-2.3 ng/ml (p<0.05). We next measured the serum MIF contents of patients with autoimmune diseases, and found that MIF levels were significantly elevated in patients with systemic lupus erythematosus and rheumatoid arthritis, 20.0+/-11.0 ng/ml and 21. 7+/-11.2 ng/ml, respectively. Using anti-MIF antibody-immobilized sepharose column chromatography, we discovered for the first time that MIF was present in erythrocytes. Taken together these results suggest that MIF plays a major role in autoimmune diseases and, moreover, potentially induces various patho-logical outcomes in cases of hemolytic disorders.
Journal Cover

April 2000
Volume 5 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

2013 Impact Factor: 1.88
Ranked #27/122 Medicine Research and Experimental
(total number of cites)

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APA
Mizue, Y., Nishihira, J., Miyazaki, T., Fujiwara, S., Chida, M., Nakamura, K., Kikuchi, K., & Mukai, M. (2000). Quantitation of macrophage migration inhibitory factor (MIF) using the one-step sandwich enzyme immunosorbent assay: elevated serum MIF concentrations in patients with autoimmune diseases and identification of MIF in erythrocytes.. International Journal of Molecular Medicine, 5(4), 397-800.
MLA
Mizue, Nishihira, Miyazaki, Fujiwara, Chida, Nakamura, Kikuchi, and M Mukai. "Quantitation of macrophage migration inhibitory factor (MIF) using the one-step sandwich enzyme immunosorbent assay: elevated serum MIF concentrations in patients with autoimmune diseases and identification of MIF in erythrocytes.." International Journal of Molecular Medicine International Journal of Molecular Medicine 5.4 (2000): 397-800.
Chicago
Mizue, Nishihira, Miyazaki, Fujiwara, Chida, Nakamura, Kikuchi, and M Mukai. "Quantitation of macrophage migration inhibitory factor (MIF) using the one-step sandwich enzyme immunosorbent assay: elevated serum MIF concentrations in patients with autoimmune diseases and identification of MIF in erythrocytes.." International Journal of Molecular Medicine International Journal of Molecular Medicine 5 no. 4 (2000): 397-800.