Green tea polyphenol epigallocatechin inhibits DNA replication and consequently induces leukemia cell apoptosis

  • Authors: David M. Smith, Q. Ping Dou
  • View Affiliations

  • Published online on: Friday, June 1, 2001
  • Pages: 645-652
  • DOI: 10.3892/ijmm.7.6.645

Abstract

Consistent with the putative role of green tea in cancer prevention, tea polyphenols have previously been shown to inhibit tumor cell proliferation by inducing G1 or G2/M cell cycle arrests, also documented is their ability to induce apoptosis (programmed cell death). However, it is unclear whether or not the cell cycle effects of polyphenols are related to their cell death-inducing ability. Here we report that the tea polyphenol (-)-epigallocatechin (EGC) inhibits DNA replication in three leukemia cancer cell lines, Jurkat T, HL-60 and K562. Among all the tested tea polyphenols, EGC was found to be the most potent in accumulation of S phase cells and inhibition of the S-G2 progression. In addition, EGC-mediated inhibition of S phase progression results in induction of apoptosis, as determined by sub-G1 cell population, breakage of endonuclear DNA, cleavage of poly(ADP-ribose) polymerase and loss of cell viability. When used in cells containing low S and high G1 and G2/M populations, EGC did not induce apoptosis. Furthermore, EGC did not inhibit M-G1 transition. Our finding that EGC inhibits S phase progression that results in leukemia cell death provides a novel and plausible molecular mechanism for how green tea may inhibit the growth of rapidly proliferating neoplastic cells.
Journal Cover

June 2001
Volume 7 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

2012 Impact Factor: 1.957
Ranked #26/121 Medicine Research and Experimental
(total number of cites)

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APA
Smith, D., & Dou, Q. (2001). Green tea polyphenol epigallocatechin inhibits DNA replication and consequently induces leukemia cell apoptosis. International Journal of Molecular Medicine, 7(6), 645-652.
MLA
Smith, and Q. Dou. "Green tea polyphenol epigallocatechin inhibits DNA replication and consequently induces leukemia cell apoptosis." International Journal of Molecular Medicine International Journal of Molecular Medicine 7.6 (2001): 645-652.
Chicago
Smith, and Q. Dou. "Green tea polyphenol epigallocatechin inhibits DNA replication and consequently induces leukemia cell apoptosis." International Journal of Molecular Medicine International Journal of Molecular Medicine 7 no. 6 (2001): 645-652.