Expression and interaction of different catenins in colorectal carcinoma cells

  • Authors: D. Kucerova, E. Sloncova, Z. Tuhackova, M. Vojtechova, V. Sovova
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  • Published online on: Saturday, December 1, 2001
  • Pages: 695-698
  • DOI: 10.3892/ijmm.8.6.695

Abstract

Aberrant signalling activities of β-catenin, originally identified as a component of cell-adhesion complexes, are now considered to be an important factor in colorectal carcinogenesis. However, recently it was shown that also γ- as well as p120 catenins have a dual role either in cell adhesion or in affecting some gene activation. Therefore, the levels and interactions of these three catenins in human colorectal carcinoma cell lines were analysed. A great heterogeneity in the expression of all catenins tested was found in colorectal carcinoma cell lines HT29 and LS174T. Detailed analysis of β-catenin interactions was done. GST-APC fragment-fused proteins were used to absorb β-catenin and its complexes from cell lysates. Similarly, the E-cadherin binding capacity of the residual pool of β-catenin was analysed using the GST-ECT construct. It was found that the level of β-catenin does not necessarily depend either on the APC or β-catenin gene mutations and that co-precipitation of β-, γ-, and p120 catenins is not limited to cells that express E-cadherin.
Journal Cover

December 2001
Volume 8 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

2012 Impact Factor: 1.88
Ranked #27/122 Medicine Research and Experimental
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APA
Kucerova, D., Sloncova, E., Tuhackova, Z., Vojtechova, M., & Sovova, V. (2001). Expression and interaction of different catenins in colorectal carcinoma cells. International Journal of Molecular Medicine, 8(6), 695-698.
MLA
Kucerova, Sloncova, Tuhackova, Vojtechova, and V. Sovova. "Expression and interaction of different catenins in colorectal carcinoma cells." International Journal of Molecular Medicine International Journal of Molecular Medicine 8.6 (2001): 695-698.
Chicago
Kucerova, Sloncova, Tuhackova, Vojtechova, and V. Sovova. "Expression and interaction of different catenins in colorectal carcinoma cells." International Journal of Molecular Medicine International Journal of Molecular Medicine 8 no. 6 (2001): 695-698.