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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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January 1997 Volume 10 Issue 1

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

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January 1997 Volume 10 Issue 1

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Article

Biochemical properties of Xenopus laevis p53

  • Authors:
    • M Hoever
    • C Herrmann
    • M Montenarh
  • View Affiliations / Copyright

    Affiliations: UNIV SAARLAND,D-66421 HOMBURG,GERMANY.
  • Pages: 195-203
    |
    Published online on: January 1, 1997
       https://doi.org/10.3892/ijo.10.1.195
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Abstract

The p53 gene has evolutionarily highly conserved boxes from rainbow trout to man whereas other regions are divergent in different species. In order to define conserved and species specific properties of p53 we analyzed frog p53. Since the commonly available monoclonal antibodies do not react with Xenopus p53 we expressed a recently cloned Xenopus laevis cDNA in bacteria and this protein was used to generate polyclonal antibodies. This antibody recognizes p53 from Xenopus laevis oocytes, baculovirus infected insect cells and p53 from an in vitro transcription/translation reaction but not from somatic Xenopus tissue cells. In contrast to p53 from other species Xenopus p53 is mainly found in the cytoplasm. Xenopus p53 forms mainly tetramers as p53 from other species. Like p53 from other species Xenopus p53 is tightly associated with a protein kinase activity and phosphorylated by this associated enzyme. Xenopus p53 binds strongly to its own RNA without any influence on the translation of p53 mRNA. Thus, Xenopus p53 seems to have some properties in common with p53 from other species and some additional species specific features.

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Copy and paste a formatted citation
Spandidos Publications style
Hoever M, Herrmann C and Montenarh M: Biochemical properties of Xenopus laevis p53. Int J Oncol 10: 195-203, 1997.
APA
Hoever, M., Herrmann, C., & Montenarh, M. (1997). Biochemical properties of Xenopus laevis p53. International Journal of Oncology, 10, 195-203. https://doi.org/10.3892/ijo.10.1.195
MLA
Hoever, M., Herrmann, C., Montenarh, M."Biochemical properties of Xenopus laevis p53". International Journal of Oncology 10.1 (1997): 195-203.
Chicago
Hoever, M., Herrmann, C., Montenarh, M."Biochemical properties of Xenopus laevis p53". International Journal of Oncology 10, no. 1 (1997): 195-203. https://doi.org/10.3892/ijo.10.1.195
Copy and paste a formatted citation
x
Spandidos Publications style
Hoever M, Herrmann C and Montenarh M: Biochemical properties of Xenopus laevis p53. Int J Oncol 10: 195-203, 1997.
APA
Hoever, M., Herrmann, C., & Montenarh, M. (1997). Biochemical properties of Xenopus laevis p53. International Journal of Oncology, 10, 195-203. https://doi.org/10.3892/ijo.10.1.195
MLA
Hoever, M., Herrmann, C., Montenarh, M."Biochemical properties of Xenopus laevis p53". International Journal of Oncology 10.1 (1997): 195-203.
Chicago
Hoever, M., Herrmann, C., Montenarh, M."Biochemical properties of Xenopus laevis p53". International Journal of Oncology 10, no. 1 (1997): 195-203. https://doi.org/10.3892/ijo.10.1.195
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