Detection of differentially expressed genes in human bladder cancer cells using arbitrarily primed PCR of RNA

  • Authors:
    • S Maack
    • R Knuechel
    • F Hofstaedter
    • A Stark
    • J Schlegel
  • Corresponding author:
  • View Affiliations

  • Published online on: Friday, August 1, 1997
  • Pages: 383-387 DOI: 10.3892/ijo.11.2.383

Abstract

The aim of the present study was to detect differentially expressed genes in human bladder cancer cell lines using a non-radioactive RNA fingerprinting technique (arbitrarily primed polymerase chain reaction of RNA, RAP-PCR). The two clonal urothelial cancer cell lines, RT4 and J82, show different growth kinetics upon stimulation with EGF. By RAP-PCR we detected changes in band patterns for J82 cells treated with EGF but not for RT4 cells. Polymorphic fragments were further characterized and sequences from two of these gave a perfect match to the coding sequence of the human tropomyosin gene TM30(pl) and the human MAC30 gene, respectively. In accordance with the results of RAP-PCR downregulation in EGF-stimulated J82 cells could be demonstrated by reverse transcription PCR.
Journal Cover

August 1997
Volume 11 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

2013 Impact Factor: 2.773
2014 I.F. (Expected) ≥ 3.225 Ranked #30/202 Oncology
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APA
Maack, S., Knuechel, R., Hofstaedter, F., Stark, A., & Schlegel, J. (1997). Detection of differentially expressed genes in human bladder cancer cells using arbitrarily primed PCR of RNA. International Journal of Oncology, 11, 383-387. http://dx.doi.org/10.3892/ijo.11.2.383
MLA
Maack, S., Knuechel, R., Hofstaedter, F., Stark, A., Schlegel, J."Detection of differentially expressed genes in human bladder cancer cells using arbitrarily primed PCR of RNA". International Journal of Oncology 11.2 (1997): 383-387.
Chicago
Maack, S., Knuechel, R., Hofstaedter, F., Stark, A., Schlegel, J."Detection of differentially expressed genes in human bladder cancer cells using arbitrarily primed PCR of RNA". International Journal of Oncology 11, no. 2 (1997): 383-387. http://dx.doi.org/10.3892/ijo.11.2.383