Detection of differentially expressed genes in human bladder cancer cells using arbitrarily primed PCR of RNA

  • Authors: S Maack, R Knuechel, F Hofstaedter, A Stark, J Schlegel
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  • Published online on: Friday, August 1, 1997
  • Pages: 383-387
  • DOI: 10.3892/ijo.11.2.383

Abstract

The aim of the present study was to detect differentially expressed genes in human bladder cancer cell lines using a non-radioactive RNA fingerprinting technique (arbitrarily primed polymerase chain reaction of RNA, RAP-PCR). The two clonal urothelial cancer cell lines, RT4 and J82, show different growth kinetics upon stimulation with EGF. By RAP-PCR we detected changes in band patterns for J82 cells treated with EGF but not for RT4 cells. Polymorphic fragments were further characterized and sequences from two of these gave a perfect match to the coding sequence of the human tropomyosin gene TM30(pl) and the human MAC30 gene, respectively. In accordance with the results of RAP-PCR downregulation in EGF-stimulated J82 cells could be demonstrated by reverse transcription PCR.
Journal Cover

August 1997
Volume 11 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

2012 Impact Factor: 2.773
Ranked #30/202 Oncology
(total number of cites)

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APA
Maack, S., Knuechel, R., Hofstaedter, F., Stark, A., & Schlegel, J. (1997). Detection of differentially expressed genes in human bladder cancer cells using arbitrarily primed PCR of RNA. International Journal of Oncology, 11(2), 383-387.
MLA
Maack, Knuechel, Hofstaedter, Stark, and J Schlegel. "Detection of differentially expressed genes in human bladder cancer cells using arbitrarily primed PCR of RNA." International Journal of Oncology International Journal of Oncology 11.2 (1997): 383-387.
Chicago
Maack, Knuechel, Hofstaedter, Stark, and J Schlegel. "Detection of differentially expressed genes in human bladder cancer cells using arbitrarily primed PCR of RNA." International Journal of Oncology International Journal of Oncology 11 no. 2 (1997): 383-387.