|Detection of differentially expressed genes in human bladder cancer cells using arbitrarily primed PCR of RNA|
Authors: S Maack, R Knuechel, F Hofstaedter, A Stark, J Schlegel
Affiliations: UNIV MARBURG,MED CTR,DEPT NEUROPATHOL,D-35043 MARBURG,GERMANY. UNIV REGENSBURG,INST PATHOL,D-93053 REGENSBURG,GERMANY.
The aim of the present study was to detect differentially expressed genes in human bladder cancer cell lines using a non-radioactive RNA fingerprinting technique (arbitrarily primed polymerase chain reaction of RNA, RAP-PCR). The two clonal urothelial cancer cell lines, RT4 and J82, show different growth kinetics upon stimulation with EGF. By RAP-PCR we detected changes in band patterns for J82 cells treated with EGF but not for RT4 cells. Polymorphic fragments were further characterized and sequences from two of these gave a perfect match to the coding sequence of the human tropomyosin gene TM30(pl) and the human MAC30 gene, respectively. In accordance with the results of RAP-PCR downregulation in EGF-stimulated J82 cells could be demonstrated by reverse transcription PCR.