Expressions of epidermal growth factor family and its receptor in hepatocellular carcinoma cell lines: relationship to cell proliferation.

  • Authors:
    • T Hisaka
    • H Yano
    • M Haramaki
    • I Utsunomiya
    • M Kojiro
  • View Affiliations

  • Published online on: Monday, March 1, 1999
  • Pages: 453-513
  • DOI: 10.3892/ijo.14.3.453

Abstract

In 6 HCC cell lines, clear expressions of EGFR and TGF-alpha were found in flow cytometry, while expressions of EGF, HB-EGF and AR were quite low. TGF-alpha secretion into culture supernatants became measurable when TPA 0.5 microM was added. TPA accelerated the proliferation of KYN-3 cells, and anti-TGF-alpha neutralizing antibody suppressed this proliferation in a dose-dependent manner. Addition of exogenous TGF-alpha, EGF, AR, or HB-EGF with heparin accelerated cell proliferation. In non-stimulated cultures, cell proliferation was suppressed by anti-EGFR neutralizing antibody, but not by the antibodies for EGF, TGF-alpha, AR and HB-EGF. HCC may possess a paracrine system regulated by these 4 ligands, and an autocrine system, under a certain condition, via TGF-alpha and EGFR.
Journal Cover

March 1999
Volume 14 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

2013 Impact Factor: 2.773
Ranked #30/202 Oncology
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APA
Hisaka, T., Yano, H., Haramaki, M., Utsunomiya, I., & Kojiro, M. (1999). Expressions of epidermal growth factor family and its receptor in hepatocellular carcinoma cell lines: relationship to cell proliferation.. International Journal of Oncology, 14(3), 453-513.
MLA
Hisaka, Yano, Haramaki, Utsunomiya, and M Kojiro. "Expressions of epidermal growth factor family and its receptor in hepatocellular carcinoma cell lines: relationship to cell proliferation.." International Journal of Oncology International Journal of Oncology 14.3 (1999): 453-513.
Chicago
Hisaka, Yano, Haramaki, Utsunomiya, and M Kojiro. "Expressions of epidermal growth factor family and its receptor in hepatocellular carcinoma cell lines: relationship to cell proliferation.." International Journal of Oncology International Journal of Oncology 14 no. 3 (1999): 453-513.