Expressions of epidermal growth factor family and its receptor in hepatocellular carcinoma cell lines: relationship to cell proliferation.

  • Authors:
    • T Hisaka
    • H Yano
    • M Haramaki
    • I Utsunomiya
    • M Kojiro
  • View Affiliations

  • Published online on: March 1, 1999     https://doi.org/10.3892/ijo.14.3.453
  • Pages: 453-513
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Abstract

In 6 HCC cell lines, clear expressions of EGFR and TGF-alpha were found in flow cytometry, while expressions of EGF, HB-EGF and AR were quite low. TGF-alpha secretion into culture supernatants became measurable when TPA 0.5 microM was added. TPA accelerated the proliferation of KYN-3 cells, and anti-TGF-alpha neutralizing antibody suppressed this proliferation in a dose-dependent manner. Addition of exogenous TGF-alpha, EGF, AR, or HB-EGF with heparin accelerated cell proliferation. In non-stimulated cultures, cell proliferation was suppressed by anti-EGFR neutralizing antibody, but not by the antibodies for EGF, TGF-alpha, AR and HB-EGF. HCC may possess a paracrine system regulated by these 4 ligands, and an autocrine system, under a certain condition, via TGF-alpha and EGFR.

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Mar 1999
Volume 14 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Hisaka T, Yano H, Haramaki M, Utsunomiya I and Kojiro M: Expressions of epidermal growth factor family and its receptor in hepatocellular carcinoma cell lines: relationship to cell proliferation.. Int J Oncol 14: 453-513, 1999.
APA
Hisaka, T., Yano, H., Haramaki, M., Utsunomiya, I., & Kojiro, M. (1999). Expressions of epidermal growth factor family and its receptor in hepatocellular carcinoma cell lines: relationship to cell proliferation.. International Journal of Oncology, 14, 453-513. https://doi.org/10.3892/ijo.14.3.453
MLA
Hisaka, T., Yano, H., Haramaki, M., Utsunomiya, I., Kojiro, M."Expressions of epidermal growth factor family and its receptor in hepatocellular carcinoma cell lines: relationship to cell proliferation.". International Journal of Oncology 14.3 (1999): 453-513.
Chicago
Hisaka, T., Yano, H., Haramaki, M., Utsunomiya, I., Kojiro, M."Expressions of epidermal growth factor family and its receptor in hepatocellular carcinoma cell lines: relationship to cell proliferation.". International Journal of Oncology 14, no. 3 (1999): 453-513. https://doi.org/10.3892/ijo.14.3.453