p53 in SV40-transformed DNA-damaged human cells binds to its cognate sequence but fails to transactivate target genes

  • Authors:
    • Antje Technau
    • Armin Wolff
    • Christian Sauder
    • Nico Birkner
    • Gerhard Brandner
  • View Affiliations

  • Published online on: February 1, 2001     https://doi.org/10.3892/ijo.18.2.281
  • Pages: 281-286
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Abstract

Human SV40-transformed cells contain high levels of stabilized p53 of which only a fraction is complexed with the SV40 large tumor antigen (T-antigen). This raises the question whether the p53 which is not complexed with T-antigen retains some biological activity. Two human SV40-transformed cell lines, BEAS and SV80, were investigated. A significant level of constitutive cognate-sequence-specific DNA-binding of p53 was detected by electrophoretic mobility shift assay (EMSA) of cell extracts. Upon DNA damage by treatment with mitomycin C the DNA-binding activity was increased, as known for cells with wild-type p53. However, in both cell lines, before and after DNA damage, p53 was not able to transactivate a target gene as shown by reporter gene assay. Hence, the capability of p53 to bind its cognate sequence is a prerequisite but no proof of p53 transactivating activity. Nuclear p53 levels were not further increased after mitomycin C treatment, occasionally rather slightly decreased, often accompanied by an even larger decrease in amount of T-antigen. In conclusion, SV40-transformation of human cells has caused a loss of essential features of wild-type p53 activity, even in that fraction of p53 not in physical complex with SV40 T-antigen.

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February 2001
Volume 18 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Technau A, Wolff A, Sauder C, Birkner N and Brandner G: p53 in SV40-transformed DNA-damaged human cells binds to its cognate sequence but fails to transactivate target genes. Int J Oncol 18: 281-286, 2001.
APA
Technau, A., Wolff, A., Sauder, C., Birkner, N., & Brandner, G. (2001). p53 in SV40-transformed DNA-damaged human cells binds to its cognate sequence but fails to transactivate target genes. International Journal of Oncology, 18, 281-286. https://doi.org/10.3892/ijo.18.2.281
MLA
Technau, A., Wolff, A., Sauder, C., Birkner, N., Brandner, G."p53 in SV40-transformed DNA-damaged human cells binds to its cognate sequence but fails to transactivate target genes". International Journal of Oncology 18.2 (2001): 281-286.
Chicago
Technau, A., Wolff, A., Sauder, C., Birkner, N., Brandner, G."p53 in SV40-transformed DNA-damaged human cells binds to its cognate sequence but fails to transactivate target genes". International Journal of Oncology 18, no. 2 (2001): 281-286. https://doi.org/10.3892/ijo.18.2.281