Characterization of novel cell lines established from three human oral squamous cell carcinomas

Lee,Gene; Kim,Youn-Bae; Kim,Jin Hong; Kim,Myung-Soo; Shin,Ki-Hyuk; Won,Young Suk; Lee,Jae-Il; Choung,Pill-Hoon; Hyun,Byung-Hwa; Min,Byung-Moo

doi:10.3892/ijo.20.6.1151

June 2002 Volume 20 Issue 6

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June 2002 Volume 20 Issue 6

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Article

Characterization of novel cell lines established from three human oral squamous cell carcinomas

Authors:
- Gene Lee
- Youn-Bae Kim
- Jin Hong Kim
- Myung-Soo Kim
- Ki-Hyuk Shin
- Young Suk Won
- Jae-Il Lee
- Pill-Hoon Choung
- Byung-Hwa Hyun
- Byung-Moo Min
View Affiliations / Copyright

Affiliations: Department of Oral Biochemistry, College of Dentistry Seoul National University, Seoul, Korea
Pages: 1151-1159
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Published online on: June 1, 2002

https://doi.org/10.3892/ijo.20.6.1151
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Abstract

Human oral squamous cell carcinoma cell lines (KOSCC-11, -25A, -25B, -25C, -25D, -25E, -33A, and -33B) were established by explantation culture from these oral squamous cell carcinomas. The histopathology of the primary tumors, in vitro growth characteristics, epithelial origin, in vitro anchorage-independency, in vivo tumorigenicity, the frequency of human papillomavirus (HPV) infections, and the status of proto-oncogenes, tumor suppressor genes, DNA mismatch repair genes, and microsatellite instability were investigated in the cell lines. KOSCC-11 is a well-differentiated oral squamous cell carcinoma (OSCC) derived from mandibular gingiva. KOSCC-25A, -25B, -25C, -25D, and -25E cell lines were derived from the same OSCC. KOSCC-33A and -33B were established from the same tumor that originated from the maxillary sinus. All tumor lines studied grew as monolayers and showed: i) epithelial origin by the presence of desmosome and keratin; ii) in vitro anchorage-independent growth ability; and iii) tumorigenic potential in nude mice. The cancer cell lines did not contain HPV DNA and did not express viral genes. Northern blot analysis revealed: i) overexpression of EGFR in four cell lines, ii) overexpression of c-H-ras in four cell lines, iii) overexpression of c-myc in three cell lines, iv) decreased expression of TGF-α in seven cell lines, and v) decreased expression of c-jun in five cancer cell lines compared with normal human oral keratinocytes. In all KOSCC cell lines and their corresponding tumor tissues, mutations were identified in highly-conserved functional regions of the p53 gene. The KOSCC-11 cell line contained a frameshift mutation and the other cell lines harbored an identical p53 mutation at codon 175 from CGC (Arg) to CTC (Leu). In five cell lines, a significant reduction of p21WAF1/Cip1 protein was evident. Cancer cell lines expressed higher level of Rb protein than normal human oral keratinocytes. DCC, a tumor suppressor gene, was not detected in KOSCC-25C. The KOSCC-33A cell line displayed microsatellite instability and showed a loss of hMSH2 expression. These well-characterized human OSCC cell lines should serve as useful tools for understanding the biological characteristics of oral cancer.

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Characterization of novel cell lines established from three human oral squamous cell carcinomas

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Abstract

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