Gene expression profiling of human HBV- and/or HCV-associated hepatocellular carcinoma cells using expressed sequence tags

  • Authors:
    • Sun Young Yoon
    • Jeong-Min Kim
    • Jung-Hwa Oh
    • Yeo-Jin Jeon
    • Dong-Seok Lee
    • Joo Heon Kim
    • Jong Young Choi
    • Byung Min Ahn
    • Sangsoo Kim
    • Hyang-Sook Yoo
    • Yong Sung Kim
    • Nam-Soon Kim
  • View Affiliations

  • Published online on: Tuesday, August 1, 2006
  • Pages: 315-327
  • DOI: 10.3892/ijo.29.2.315

Abstract

Liver cancer is one of the leading causes of cancer death worldwide. To identify novel target genes that are related to liver carcinogenesis, we examined new genes that are differentially expressed in human hepatocellular carcinoma (HCC) cell lines and tissues based on the expressed sequence tag (EST) frequency. Eleven libraries were constructed from seven HCC cell lines and three normal liver tissue samples obtained from Korean patients. An analysis of gene expression profiles for HCC was performed using the frequency of ESTs obtained from these cDNA libraries. Genes were identified (n=120) as being either up- or down-regulated in human liver cancer cells. Among these, 14 genes (FTL, K-ALPHA1, LDHA, RPL4, ENO1, ANXA2, RPL9, RPL10, RPL13A, GNB2L1, AMBP, GC, A1BG, and SERPINC1), in addition to previously well-known liver cancer related genes, were confirmed to be differentially expressed in seven liver cancer cell lines and 17 HCC tissues by semi-quantitative RT-PCR. In addition, 73 genes, in which there was a significant difference (P>0.99) between HBV- and HCV-associated HCC cells, were selected. Of these, expression patterns of 14 (RPLP0, AKR1C, KRT8, GPX4, RPS15, ID1, RPS21, VIM, EEF1G, EIF4A1, HLA-C, FN1, CD44, and RPS10) were confirmed by semi-quantitative RT-PCR in four of HBV- and three of HCV-associated HCC cell lines. Among those genes, an immunohistochemical analysis for ANXA2 showed that it is expressed at high levels in HCC. Using an analysis of EST frequency, the newly identified genes, especially ANXA2, represent potential biomarkers for HCC and useful targets for elucidating the molecular mechanisms associated with HCC involving virological etiology.
Journal Cover

August 2006
Volume 29 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

2013 Impact Factor: 2.773
Ranked #30/202 Oncology
(total number of cites)

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Yoon, S., Kim, J., Oh, J., Jeon, Y., Lee, D., Kim, J., Choi, J., Ahn, B., Kim, S., Yoo, H., Kim, Y., & Kim, N. (2006). Gene expression profiling of human HBV- and/or HCV-associated hepatocellular carcinoma cells using expressed sequence tags. International Journal of Oncology, 29(2), 315-327.
MLA
Yoon, Kim, Oh, Jeon, Lee, Kim, Choi, Ahn, Kim, Yoo, Kim, and Nam-Soon Kim. "Gene expression profiling of human HBV- and/or HCV-associated hepatocellular carcinoma cells using expressed sequence tags." International Journal of Oncology International Journal of Oncology 29.2 (2006): 315-327.
Chicago
Yoon, Kim, Oh, Jeon, Lee, Kim, Choi, Ahn, Kim, Yoo, Kim, and Nam-Soon Kim. "Gene expression profiling of human HBV- and/or HCV-associated hepatocellular carcinoma cells using expressed sequence tags." International Journal of Oncology International Journal of Oncology 29 no. 2 (2006): 315-327.