Control of cellular redox status and upregulation of quinone reductase NQO1 via Nrf2 activation by α-lipoic acid in human leukemia HL-60 cells

  • Authors:
    • Selvakumar Elangovan
    • Tze-Chen Hsieh
  • View Affiliations

  • Published online on: Wednesday, October 1, 2008
  • Pages: 833-838
  • DOI: 10.3892/ijo_00000071

Abstract

α-Lipoic acid (LA) is a naturally-occurring micronutrient that has been actively investigated for the treatment and management of various chronic medical conditions including neurodegenerative diseases, diabetes and hepatic disorders. However, relatively few studies have examined the effects of LA as a chemopreventive agent, particularly in regard to its ability to modulate homeostasis of oxidoreductive state and to regulate detoxification enzymes such as quinone reductase NQO1 in LA-responsive cells. We tested the hypothesis that LA affects the intracellular redox status and induces NQO1 expression using the human promyelocytic HL-60 leukemia cells. We showed that treatment by LA maintains HL-60 cells in a relatively reduced state, supported by the dose/time-dependent increase in the activities of glutathione peroxidase and glutathione reductase and decrease in the activity of catalase. Moreover, LA significantly increased the activity and protein expression of NQO1. The induction of NQO1 was accompanied by the nuclear accumulation of transcription factor Nrf2, which was correlated with a decreased level of Nrf2 in the cytosol as well as the concomitant reduction in the expression of cytoplasmic repressor of Nrf2, Keap1.
Journal Cover

October 2008
Volume 33 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

2013 Impact Factor: 2.773
Ranked #30/202 Oncology
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APA
Elangovan, S., & Hsieh, T. (2008). Control of cellular redox status and upregulation of quinone reductase NQO1 via Nrf2 activation by α-lipoic acid in human leukemia HL-60 cells. International Journal of Oncology, 33(4), 833-838.
MLA
Elangovan, and Tze-Chen Hsieh. "Control of cellular redox status and upregulation of quinone reductase NQO1 via Nrf2 activation by α-lipoic acid in human leukemia HL-60 cells." International Journal of Oncology International Journal of Oncology 33.4 (2008): 833-838.
Chicago
Elangovan, and Tze-Chen Hsieh. "Control of cellular redox status and upregulation of quinone reductase NQO1 via Nrf2 activation by α-lipoic acid in human leukemia HL-60 cells." International Journal of Oncology International Journal of Oncology 33 no. 4 (2008): 833-838.