Differential effects of malignant mesothelioma cells on THP-1 monocytes and macrophages

  • Authors:
    • Valerio Izzi
    • Valerio Chiurchiù
    • Fabiola D'Aquilio
    • Camilla Palumbo
    • Ilaria Tresoldi
    • Andrea Modesti
    • Patrizia M. Baldini
  • View Affiliations

  • Published online on: Sunday, February 1, 2009
  • Pages: 543-550
  • DOI: 10.3892/ijo_00000180

Abstract

Malignant mesothelioma (MM) is a highly fatal tumor arising from inner body membranes, whose extensive growth is facilitated by its week immunogenicity and by its ability to blunt the immune response which should arise from the huge mass of leukocytes typically infiltrating this tumor. It has been reported that the inflammatory infiltrate found in MM tissues is characterized by a high prevalence of macrophages. Thus, in this work we evaluated the ability of human MM cells to modulate the inflammatory phenotype of human THP-1 monocytes and macrophages, a widely used in vitro model of monocyte/macrophage differentiation. Furthermore, we tested the hypothesis that the exposure to MM cells could alter the differentiation of THP-1 monocytes favoring the development of alternatively activated, tumor-supporting macrophages. Our data prove for the first time that MM cells can polarize monocytes towards an altered inflammatory phenotype and macrophages towards an immunosuppressive phenotype. Moreover, we demonstrate that monocytes cocultivated with MM cells ‘keep a memory’ of their encounter with the tumor which influences their differentiation to macrophages. On the whole, we provide evidence that MM cells exert distinct, cell-specific effects on monocytes and macrophages. The thorough characterization of such effects may be of a crucial importance for the rational design of new immunotherapeutic protocols.
Journal Cover

February 2009
Volume 34 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

2013 Impact Factor: 2.773
Ranked #30/202 Oncology
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APA
Izzi, V., Chiurchiù, V., D'Aquilio, F., Palumbo, C., Tresoldi, I., Modesti, A., & Baldini, P. (2009). Differential effects of malignant mesothelioma cells on THP-1 monocytes and macrophages. International Journal of Oncology, 34(2), 543-550.
MLA
Izzi, Chiurchiù, D'Aquilio, Palumbo, Tresoldi, Modesti, and Patrizia Baldini. "Differential effects of malignant mesothelioma cells on THP-1 monocytes and macrophages." International Journal of Oncology International Journal of Oncology 34.2 (2009): 543-550.
Chicago
Izzi, Chiurchiù, D'Aquilio, Palumbo, Tresoldi, Modesti, and Patrizia Baldini. "Differential effects of malignant mesothelioma cells on THP-1 monocytes and macrophages." International Journal of Oncology International Journal of Oncology 34 no. 2 (2009): 543-550.