Overexpression of eukaryotic elongation factor eEF2 in gastrointestinal cancers and its involvement in G2/M progression in the cell cycle

  • Authors: Junya Nakamura, Sayaka Aoyagi, Isamu Nanchi, Shin-Ichi Nakatsuka, Erika Hirata, Shohei Shibata, Mari Fukuda, Yumiko Yamamoto, Ikuyo Fukuda, Naoya Tatsumi, Tazu Ueda, Fumihiro Fujiki, Masaya Nomura, Sumiyuki Nishida, Toshiaki Shirakata, Naoki Hosen, Akihoro Tsuboi, Yoshihiro Oka, Riichiro Nezu, Masaki Mori, Yuichiro Doki, Katsuyuki Aozasa, Haruo Sugiyama, Yusuke Oji
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  • Published online on: Friday, May 1, 2009
  • Pages: 1181-1189
  • DOI: 10.3892/ijo_00000246

Abstract

A high level protein synthesis is one of the characteristics of cancer cells. The aim of this study is to show the contribution of eukaryotic elongation factor 2 (eEF2), which plays an essential role in the polypeptide chain elongation step, in the tumorigenesis of gastrointestinal cancers. In the present study, we demonstrated by using immunohistochemistry that eEF2 protein was overexpressed in 92.9% (13 of 14) of gastric and 91.7% (22 of 24) of colorectal cancers. No mutations were found in any of the exons of the eEF2 gene in six gastric and six colorectal cancers. Knockdown of eEF2 by eEF2-specific short-hairpin RNA (shEF2) inhibited cancer cell growth in two gastric cancer cell lines, AZ-521 and MKN28, and one colon cancer cell line, SW620. Flow cytometric analysis showed that knockdown of eEF2 induced G2/M arrest and resulted in inactivation of Akt and cdc2 (a G2/M regulator) and activation of eEF2 kinase (a negative regulator of eEF2) in these cancer cells. Conversely, forced expression of eEF2 in AZ-521 cells significantly enhanced the cell growth through promotion of G2/M progression in cell cycle, activated Akt and cdc2, and inactivated eEF2 kinase. Furthermore, forced expression of eEF2 in these cancer cells enhanced in vivo tumorigenicity in a mouse xenograft model. These results showed that overexpressed eEF2 in gastrointestinal cancers promoted G2/M progression and enhanced their cell growth in vitro and in vivo. These results also suggested a novel linkage between translational elongation and cell cycle mechanisms, implying that the linkage might play an important role to orchestrate the deregulated translation and cell cycle mechanisms for promotion of the development of gastrointestinal cancers.
Journal Cover

May 2009
Volume 34 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

2012 Impact Factor: 2.657
Ranked #31/196 Oncology
(total number of cites)

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APA
Nakamura, J., Aoyagi, S., Nanchi, I., Nakatsuka, S., Hirata, E., Shibata, S., Fukuda, M., Yamamoto, Y., Fukuda, I., Tatsumi, N., Ueda, T., Fujiki, F., Nomura, M., Nishida, S., Shirakata, T., Hosen, N., Tsuboi, A., Oka, Y., Nezu, R., Mori, M., Doki, Y., Aozasa, K., Sugiyama, H., & Oji, Y. (2009). Overexpression of eukaryotic elongation factor eEF2 in gastrointestinal cancers and its involvement in G2/M progression in the cell cycle. International Journal of Oncology, 34(5), 1181-1189.
MLA
Nakamura, Aoyagi, Nanchi, Nakatsuka, Hirata, Shibata, Fukuda, Yamamoto, Fukuda, Tatsumi, Ueda, Fujiki, Nomura, Nishida, Shirakata, Hosen, Tsuboi, Oka, Nezu, Mori, Doki, Aozasa, Sugiyama, and Yusuke Oji. "Overexpression of eukaryotic elongation factor eEF2 in gastrointestinal cancers and its involvement in G2/M progression in the cell cycle." International Journal of Oncology International Journal of Oncology 34.5 (2009): 1181-1189.
Chicago
Nakamura, Aoyagi, Nanchi, Nakatsuka, Hirata, Shibata, Fukuda, Yamamoto, Fukuda, Tatsumi, Ueda, Fujiki, Nomura, Nishida, Shirakata, Hosen, Tsuboi, Oka, Nezu, Mori, Doki, Aozasa, Sugiyama, and Yusuke Oji. "Overexpression of eukaryotic elongation factor eEF2 in gastrointestinal cancers and its involvement in G2/M progression in the cell cycle." International Journal of Oncology International Journal of Oncology 34 no. 5 (2009): 1181-1189.