Sphere-forming stem-like cell populations with drug resistance in human sarcoma cell lines

  • Authors:
    • Hiromasa Fujii
    • Kanya Honoki
    • Toshifumi Tsujiuchi
    • Akira Kido
    • Kazuhiro Yoshitani
    • Yoshinori Takakura
  • Corresponding author:
  • View Affiliations

  • Published online on: Friday, May 1, 2009
  • Pages: 1381-1386 DOI: 10.3892/ijo_00000265

Abstract

The presence of cancer stem cells in both solid and hematopoietic malignancies, has been recently linked to their pathogenesis. Sarcomas are rare, and diversely characterized by degrees of mesenchymal differentiation. The aim of the current study was to demonstrate whether the human sarcoma cell lines, osteosarcoma MG63, Ewing's sarcoma HTB166, fibrosarcoma HT1080, possess the stem-like properties which may contribute to the drug-resistance. All cell lines possessed an ability to form spherical, clonal expanding colonies (sarcospheres) in anchorage-independent, serum-starved conditions. Sarcospheres showed the stem-like properties with the ability of self-renewal, and increased expression of the stem cell-related genes such as Nanog, OCT3/4 SOX2 and DNA repair enzyme genes, MLH1 and MSH2. Sarcospheres showed strong resistance to doxorubicin and cisplatin, and caffeine, a DNA repair inhibitor, enhanced the efficacy of those drugs, suggesting that the drug resistance in sarcosphere cells was partly related to the efficient DNA repair ability. These results indicate that human sarcoma cell lines contain stem-like cell populations with strong drug resistance, and DNA repair inhibitor could enhance the efficacy of chemo-drugs against sarcomas.
Journal Cover

May 2009
Volume 34 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

2013 Impact Factor: 2.773
2014 I.F. (Expected) ≥ 3.258 Ranked #30/202 Oncology
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APA
Fujii, H., Honoki, K., Tsujiuchi, T., Kido, A., Yoshitani, K., & Takakura, Y. (2009). Sphere-forming stem-like cell populations with drug resistance in human sarcoma cell lines. International Journal of Oncology, 34, 1381-1386. http://dx.doi.org/10.3892/ijo_00000265
MLA
Fujii, H., Honoki, K., Tsujiuchi, T., Kido, A., Yoshitani, K., Takakura, Y."Sphere-forming stem-like cell populations with drug resistance in human sarcoma cell lines". International Journal of Oncology 34.5 (2009): 1381-1386.
Chicago
Fujii, H., Honoki, K., Tsujiuchi, T., Kido, A., Yoshitani, K., Takakura, Y."Sphere-forming stem-like cell populations with drug resistance in human sarcoma cell lines". International Journal of Oncology 34, no. 5 (2009): 1381-1386. http://dx.doi.org/10.3892/ijo_00000265