miR-145 inhibits breast cancer cell growth through RTKN

  • Authors: Shihua Wang, Chunjing Bian, Zhuo Yang, Ye Bo, Jing Li, Lifen Zeng, Hong Zhou, Robert Chunhua Zhao
  • View Affiliations

  • Published online on: Friday, May 1, 2009
  • Pages: 1461-1466
  • DOI: 10.3892/ijo_00000275

Abstract

MicroRNAs (miRNAs) represent a class of small non-coding RNAs regulating gene expression by inducing RNA degradation or interfering with translation. Aberrant miRNA expression has been described for several human malignancies. Herein, we show that miR-145 is down-regulated in human cancer cell line MCF-7 when compared to normal human mammary epithelial cell line MCF10A. Overexpression of miR-145 by plasmid inhibits MCF-7 cell growth and induces apoptosis. Subsequently, RTKN is identified as a potential miR-145 target by bioinformatics. Using reporter constructs, we show that the RTKN 3' untranslated region (3'UTR) carries the directly binding site of miR-145. Additionally, overexpression of miR-145 in MCF-7 reduces RTKN protein expression as well as mRNA level. Furthermore, down-regulation of RTKN by siRNA can inhibit MCF-7 cell growth. Taken together, we propose that loss of miR-145 may provide a selective growth advantage for MCF-7 by targeting RTKN.
Journal Cover

May 2009
Volume 34 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

2012 Impact Factor: 2.773
Ranked #30/202 Oncology
(total number of cites)

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APA
Wang, S., Bian, C., Yang, Z., Bo, Y., Li, J., Zeng, L., Zhou, H., & Zhao, R. (2009). miR-145 inhibits breast cancer cell growth through RTKN. International Journal of Oncology, 34(5), 1461-1466.
MLA
Wang, Bian, Yang, Bo, Li, Zeng, Zhou, and Robert Zhao. "miR-145 inhibits breast cancer cell growth through RTKN." International Journal of Oncology International Journal of Oncology 34.5 (2009): 1461-1466.
Chicago
Wang, Bian, Yang, Bo, Li, Zeng, Zhou, and Robert Zhao. "miR-145 inhibits breast cancer cell growth through RTKN." International Journal of Oncology International Journal of Oncology 34 no. 5 (2009): 1461-1466.