Open Access

Analysis of a correlation between the BRAF V600E mutation and abnormal DNA mismatch repair in patients with sporadic endometrial cancer

  • Authors:
    • Makiko Kawaguchi
    • Megumi Yanokura
    • Kouji Banno
    • Yusuke Kobayashi
    • Yoshiko Kuwabara
    • Maya Kobayashi
    • Hiroyuki Nomura
    • Akira Hirasawa
    • Nobuyuki Susumu
    • Daisuke Aoki
  • View Affiliations

  • Published online on: Monday, June 1, 2009
  • Pages: 1541-1547
  • DOI: 10.3892/ijo_00000283

Abstract

Point mutations of KRAS and BRAF genes are thought to be important in carcinogenesis of colon cancer. In particular, gene instability caused by decreased expression of the hMLH1 gene, a DNA mismatch repair (MMR) gene, may be linked to the activating BRAF V600E point mutation in sporadic colon cancer. However, a consensus has not been established regarding the correlation between point mutations of KRAS or BRAF and carcinogenesis in patients with endometrial cancer, which is closely related to colon cancer. Therefore, we analyzed aberrant hypermethylation of the hMLH1 gene, microsatellite instability (MSI), and point mutations of KRAS and BRAF in 44 samples of sporadic endo-metrial cancer, with the aim of examining the mechanism of carcinogenesis in patients with endometrial cancer. Aberrant hMLH1 hypermethylation was found in 17 of the 44 cases (38.6%) and showed a significant positive correlation with MSI (p=0.02). This suggests that an abnormal MMR mechanism plays an important role in carcinogenesis of sporadic endometrial cancer. Point mutation of KRAS was found in 6 of the 44 cases (13.6%), but no BRAF V600E mutation was detected. These data suggest that the BRAF V600E mutation is not the target gene for abnormal MMR in carcinogenesis in patients with sporadic endometrial cancer, unlike in colon cancer. This is supported by the relatively few previous reports indicating a correlation between endometrial cancer and the BRAF V600E mutation. Identification of new candidates for the target gene for abnormal MMR in endometrial cancer requires further work.
Journal Cover

June 2009
Volume 34 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

2013 Impact Factor: 2.773
Ranked #30/202 Oncology
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APA
Kawaguchi, M., Yanokura, M., Banno, K., Kobayashi, Y., Kuwabara, Y., Kobayashi, M., Nomura, H., Hirasawa, A., Susumu, N., & Aoki, D. (2009). Analysis of a correlation between the BRAF V600E mutation and abnormal DNA mismatch repair in patients with sporadic endometrial cancer. International Journal of Oncology, 34(6), 1541-1547.
MLA
Kawaguchi, Yanokura, Banno, Kobayashi, Kuwabara, Kobayashi, Nomura, Hirasawa, Susumu, and Daisuke Aoki. "Analysis of a correlation between the BRAF V600E mutation and abnormal DNA mismatch repair in patients with sporadic endometrial cancer." International Journal of Oncology International Journal of Oncology 34.6 (2009): 1541-1547.
Chicago
Kawaguchi, Yanokura, Banno, Kobayashi, Kuwabara, Kobayashi, Nomura, Hirasawa, Susumu, and Daisuke Aoki. "Analysis of a correlation between the BRAF V600E mutation and abnormal DNA mismatch repair in patients with sporadic endometrial cancer." International Journal of Oncology International Journal of Oncology 34 no. 6 (2009): 1541-1547.