1,1-Bis(3'-indolyl)-1-(p-bromophenyl)methane and related compounds repress survivin and decrease γ-radiation-induced survivin in colon and pancreatic cancer cells

  • Authors: Sandeep Sreevalsan, Indira Jutooru, Gayathri Chadalapaka, Michael Walker, Stephen Safe
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  • Published online on: Sunday, November 1, 2009
  • Pages: 1191-1199
  • DOI: 10.3892/ijo_00000436

Abstract

1,1-Bis(3'-indolyl)-1-(p-bromophenyl)methane (DIM-C-pPhBr) and the 2,2'-dimethyl analog (2,2'-diMeDIM-C-pPhBr) inhibit proliferation and induce apoptosis in SW480 colon and Panc28 pancreatic cancer cells. In this study, treatment with 10-20 µM concentrations of these compounds for 24 h induced cleaved PARP and decreased survivin protein and mRNA expression in both cell lines. However, results of time course studies show that DIM-C-pPhBr and 2,2'-diMeDIM-C-pPhBr decrease survivin protein within 2 h after treatment, whereas survivin mRNA levels were decreased only at later time-points indicating activation of transcription-independent and -dependent pathways for downregulation of survivin. In addition, we also observed that γ-radiation inhibited pancreatic and colon cancer cell growth and this was associated with enhanced expression of survivin after 24 (SW480) or 24 and 48 h (Panc28) and correlated with previous studies on the role of survivin in radiation-resistance. However, in cells co-treated with γ-radiation plus DIM-C-pPhBr or 2,2'-diMeDIM-C-pPhBr, induction of survivin by γ-radiation was inhibited after co-treatment with both compounds, suggesting applications for these drugs in combination cancer chemotherapy with γ-radiation.
Journal Cover

November 2009
Volume 35 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

2013 Impact Factor: 2.773
Ranked #30/202 Oncology
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APA
Sreevalsan, S., Jutooru, I., Chadalapaka, G., Walker, M., & Safe, S. (2009). 1,1-Bis(3'-indolyl)-1-(p-bromophenyl)methane and related compounds repress survivin and decrease γ-radiation-induced survivin in colon and pancreatic cancer cells. International Journal of Oncology, 35(5), 1191-1199.
MLA
Sreevalsan, Jutooru, Chadalapaka, Walker, and Stephen Safe. "1,1-Bis(3'-indolyl)-1-(p-bromophenyl)methane and related compounds repress survivin and decrease γ-radiation-induced survivin in colon and pancreatic cancer cells." International Journal of Oncology International Journal of Oncology 35.5 (2009): 1191-1199.
Chicago
Sreevalsan, Jutooru, Chadalapaka, Walker, and Stephen Safe. "1,1-Bis(3'-indolyl)-1-(p-bromophenyl)methane and related compounds repress survivin and decrease γ-radiation-induced survivin in colon and pancreatic cancer cells." International Journal of Oncology International Journal of Oncology 35 no. 5 (2009): 1191-1199.