A vector-based short hairpin RNA targeting Aurora A inhibits breast cancer growth

  • Authors: Yang Wan, Anliang Huang, Yang Yang, Gang Xie, Xiang Chen, Jia Hu, Xiancheng Chen, Li Yang, Jiong Li, Lijuan Chen, Yu Jiang, Xia Zhao, Yuquan Wei, Hongxin Deng
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  • Published online on: Saturday, May 1, 2010
  • Pages: 1121-1128
  • DOI: 10.3892/ijo_00000594

Abstract

Aurora A plays an essential role in centrosome maturation, separation and in the formation of the mitotic bipolar spindle. Overexpression or amplication of Aurora A gene has been detected in many cancer cell lines and various tumor tissues, including breast cancer, suggesting that Aurora A might be drug target for breast cancer treatment. In the current study, short hairpin RNA targeting Aurora A was cloned into pGenesil-2 plasmid vector and then transfected into MDA-MB-435S and ZR-75-30 human breast cancer cells using cationic liposome. Reduced expression of Aurora A was detected by RT-PCR and Western blot. The effect of pGenesil-2-shAURKA plasmid on tumor growth in MDA-MB-435S xenogenic implantation model was studied. pGenesil-2-shAURKA plasmid inhibited tumor growth significantly by systemantic administration. To further study the underlying mechanisms, cell apoptosis and proliferation were investigated by flow cytometric analysis, propidium iodide staining, TUNEL and Ki-67 immunostaining respectively. Increased apoptosis and reduced cell proliferation were detected in vitro and in vivo studies. In summary, our results suggested that specific knockdown of Aurora A expression by vector based shRNA may be a potential therapy for human breast cancer.
Journal Cover

May 2010
Volume 36 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

2012 Impact Factor: 2.657
Ranked #31/196 Oncology
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APA
Wan, Y., Huang, A., Yang, Y., Xie, G., Chen, X., Hu, J., Chen, X., Yang, L., Li, J., Chen, L., Jiang, Y., Zhao, X., Wei, Y., & Deng, H. (2010). A vector-based short hairpin RNA targeting Aurora A inhibits breast cancer growth. International Journal of Oncology, 36(5), 1121-1128.
MLA
Wan, Huang, Yang, Xie, Chen, Hu, Chen, Yang, Li, Chen, Jiang, Zhao, Wei, and Hongxin Deng. "A vector-based short hairpin RNA targeting Aurora A inhibits breast cancer growth." International Journal of Oncology International Journal of Oncology 36.5 (2010): 1121-1128.
Chicago
Wan, Huang, Yang, Xie, Chen, Hu, Chen, Yang, Li, Chen, Jiang, Zhao, Wei, and Hongxin Deng. "A vector-based short hairpin RNA targeting Aurora A inhibits breast cancer growth." International Journal of Oncology International Journal of Oncology 36 no. 5 (2010): 1121-1128.