Open Access

Mapping of the interaction sites between Wee1 kinase and the regulatory β-subunit of protein kinase CK2

  • Authors:
    • Birgitte B. Olsen
    • Jan N. Kreutzer
    • Nobumoto Watanabe
    • Tina Holm
    • Barbara Guerra
  • View Affiliations

  • Published online on: Saturday, May 1, 2010
  • Pages: 1175-1182
  • DOI: 10.3892/ijo_00000600

Abstract

Somatic Wee1 is a protein kinase that plays a key role in cell cycle progression at the onset of mitosis by phosphorylating CDK1 at the inhibitory Tyr15 amino acid residue. Wee1 is regulated at multiple levels, i.e., phosphorylation, protein-protein association and proteasome-mediated degradation. We have recently shown that the regulatory β-subunit of protein kinase CK2 participates in PLK1-Wee1 complex formation interacting directly with Wee1 and thereby contributing to the regulation of G2-M cell cycle transition. Here, we show that Wee1 binds CK2β via two domains comprising amino acids 59-71 and 232-332. By employing deletion mutants of the CK2β-subunit we also show that two regions between residues 1-5 and 155-170 are necessary for binding Wee1. Furthermore, we demonstrate that the interaction between CK2β and Wee1 does not modify the kinase activity of the latter, instead CK2β directly upregulates CDK1 kinase activity by reversing the inhibitory effect which follows Wee1-mediated phosphorylation. Taken together, our findings reinforce the notion that CK2β is a modulator of protein kinases implicated in cell cycle regulation and exerts functions that are independent of CK2 tetramers.
Journal Cover

May 2010
Volume 36 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

2013 Impact Factor: 2.773
Ranked #30/202 Oncology
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APA
Olsen, B., Kreutzer, J., Watanabe, N., Holm, T., & Guerra, B. (2010). Mapping of the interaction sites between Wee1 kinase and the regulatory β-subunit of protein kinase CK2. International Journal of Oncology, 36(5), 1175-1182.
MLA
Olsen, Kreutzer, Watanabe, Holm, and Barbara Guerra. "Mapping of the interaction sites between Wee1 kinase and the regulatory β-subunit of protein kinase CK2." International Journal of Oncology International Journal of Oncology 36.5 (2010): 1175-1182.
Chicago
Olsen, Kreutzer, Watanabe, Holm, and Barbara Guerra. "Mapping of the interaction sites between Wee1 kinase and the regulatory β-subunit of protein kinase CK2." International Journal of Oncology International Journal of Oncology 36 no. 5 (2010): 1175-1182.