Cardiomyocyte differentiation of bone marrow-derived Oct-4+CXCR4+SSEA-1+ very small embryonic-like stem cells

  • Authors: Wojciech Wojakowski, Michal Tendera, Magda Kucia, Ewa Zuba-Surma, Krzysztof Milewski, David Wallace-Bradley, Maciej Kazmierski, Pawel Buszman, Eugeniusz Hrycek, Wieslaw Cybulski, Grzegorz Kaluza, Piotr Wieczorek, Janina Ratajczak, Mariusz Z. Ratajczak
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  • Published online on: Sunday, August 1, 2010
  • Pages: 237-247
  • DOI: 10.3892/ijo_00000671

Abstract

VSELs are a population of rare Oct-4+CXCR4+ CD133+lin-CD45− cells that are deposited during early embryogenesis in developing organs/tissues as a reserve population of pluripotent stem cells for regeneration. We reported recently that they are mobilized into peripheral blood during acute myocardial infarction (AMI) in mice and humans. However, although freshly isolated VSELs in experimental AMI mouse models improve cardiac function, the number of these cells is limited and an ex vivo expansion strategy is needed to employ these cells more efficiently for cardiac regeneration. The aim of this study was to establish an efficient method to expand ex vivo BM-derived very small embryonic-like stem cells (VSELs) into cardiomyocytes. VSELs, highly purified by FACS from the bone marrow of GFP transgenic mice, were expanded over C2C12 cell line myoblasts and exposed to cardiac differentiating media. The changes in gene expression during cardiac differentiation of VSELs were evaluated by RTQ-PCR, immunostaining and gene array analysis. We developed an efficient, two-step, ex vivo expansion/differentiation model of BM-derived VSELs into cardiomyocytes. First, purified GFP+ VSELs are plated over C2C12 murine cell line myoblasts, where they expand and differentiate into characteristic spheres. Subsequently, cells from these spheres are expanded on cardiac differentiating media into cardiomyocytes. This study demonstrates that murine BM-derived VSELs can be efficiently expanded in vitro and differentiated into cardiomyocytes.
Journal Cover

August 2010
Volume 37 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

2012 Impact Factor: 2.657
Ranked #31/196 Oncology
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APA
Wojakowski, W., Tendera, M., Kucia, M., Zuba-Surma, E., Milewski, K., Wallace-Bradley, D., Kazmierski, M., Buszman, P., Hrycek, E., Cybulski, W., Kaluza, G., Wieczorek, P., Ratajczak, J., & Ratajczak, M. (2010). Cardiomyocyte differentiation of bone marrow-derived Oct-4+CXCR4+SSEA-1+ very small embryonic-like stem cells. International Journal of Oncology, 37(2), 237-247.
MLA
Wojakowski, Tendera, Kucia, Zuba-Surma, Milewski, Wallace-Bradley, Kazmierski, Buszman, Hrycek, Cybulski, Kaluza, Wieczorek, Ratajczak, and Mariusz Ratajczak. "Cardiomyocyte differentiation of bone marrow-derived Oct-4+CXCR4+SSEA-1+ very small embryonic-like stem cells." International Journal of Oncology International Journal of Oncology 37.2 (2010): 237-247.
Chicago
Wojakowski, Tendera, Kucia, Zuba-Surma, Milewski, Wallace-Bradley, Kazmierski, Buszman, Hrycek, Cybulski, Kaluza, Wieczorek, Ratajczak, and Mariusz Ratajczak. "Cardiomyocyte differentiation of bone marrow-derived Oct-4+CXCR4+SSEA-1+ very small embryonic-like stem cells." International Journal of Oncology International Journal of Oncology 37 no. 2 (2010): 237-247.