Glucose metabolism of malignant cells is not regulated by transketolase-like (TKTL)-1

  • Authors: Arnulf Mayer, Angelika Von Wallbrunn, Peter Vaupel
  • View Affiliations

  • Published online on: Sunday, August 1, 2010
  • Pages: 265-271
  • DOI: 10.3892/ijo_00000674

Abstract

An isoenzyme of transketolase, transketolase-like (TKTL)-1, has been hypothesized to play a pivotal role in the pathophysiology of malignant tumors. Available data are based on the detection of the putative TKTL-1 protein with one particular mouse monoclonal anti-TKTL-1 antibody, clone JFC12T10. In this study it was demonstrated that a) JFC12T10 detects multiple unspecific bands in Western blots, b) a 75-kDa band hitherto referred to as TKTL-1 corresponds to a nuclear protein and c) immunohistochemical detection of TKTL-1 in benign leiomyomas yields an expression pattern identical to that found in a variety of malignant tumors. In RT-PCR assays, using three different primer pairs for transketolase, TKTL-1 and yet another isogene of transketolase, TKTL-2, a relevant expression of TKTL-1 was not detectable in any of the 6 malignant tumor cell lines investigated (MCF-7, A549, HeLa, HT1080, M21 and TF-1). Expression levels of TKTL-1 were rather similar to those found for TKTL-2, although the latter has never been implicated in malignant disease. On the basis of these data, nutritional recommendations based on a hypothetically TKTL-1 controlled metabolism of tumor cells must be regarded as lacking scientific evidence.
Journal Cover

August 2010
Volume 37 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

2012 Impact Factor: 2.657
Ranked #31/196 Oncology
(total number of cites)

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Mayer, A., Von Wallbrunn, A., & Vaupel, P. (2010). Glucose metabolism of malignant cells is not regulated by transketolase-like (TKTL)-1. International Journal of Oncology, 37(2), 265-271.
MLA
Mayer, Von Wallbrunn, and Peter Vaupel. "Glucose metabolism of malignant cells is not regulated by transketolase-like (TKTL)-1." International Journal of Oncology International Journal of Oncology 37.2 (2010): 265-271.
Chicago
Mayer, Von Wallbrunn, and Peter Vaupel. "Glucose metabolism of malignant cells is not regulated by transketolase-like (TKTL)-1." International Journal of Oncology International Journal of Oncology 37 no. 2 (2010): 265-271.