Establishment of in vivo fluorescence imaging in mouse models of malignant mesothelioma

  • Authors: Norie Yamaoka, Yoshiko Kawasaki, Yunfeng Xu, Hideyuki Yamamoto, Nobuyuki Terada, Haruki Okamura, Shuji Kubo
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  • Published online on: Sunday, August 1, 2010
  • Pages: 273-279
  • DOI: 10.3892/ijo_00000675

Abstract

Malignant mesothelioma is a highly aggressive tumor with poor prognosis, and new treatment paradigms are urgently needed. For testing preclinical efficacy of new therapeutic agents, establishment of appropriate animal models is crucial. We developed in vivo fluorescence imaging models for human malignant mesothelioma in mice using tumor cells engineered to express fluorescent proteins (EGFP, mRFP, mCherry, and mPlum) by lentiviral vectors. Among these fluorescent proteins, the expression of mCherry protein in the transduced tumor cells was shown to be robust and stable both in vitro and in vivo. In both, peritoneally disseminated and orthotopic pleural mesothelioma models, mCherry-positive tumors were sensitively detected and tumor growth was successfully monitored. This represents the first study to achieve sensitive tumor detection and tracking of tumor growth and development in the malignant mesothelioma mouse models by non-invasive in vivo fluorescence imaging. These imaging models can be versatile and powerful tools to explore new treatment paradigms for malignant mesothelioma.
Journal Cover

August 2010
Volume 37 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

2012 Impact Factor: 2.657
Ranked #31/196 Oncology
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APA
Yamaoka, N., Kawasaki, Y., Xu, Y., Yamamoto, H., Terada, N., Okamura, H., & Kubo, S. (2010). Establishment of in vivo fluorescence imaging in mouse models of malignant mesothelioma. International Journal of Oncology, 37(2), 273-279.
MLA
Yamaoka, Kawasaki, Xu, Yamamoto, Terada, Okamura, and Shuji Kubo. "Establishment of in vivo fluorescence imaging in mouse models of malignant mesothelioma." International Journal of Oncology International Journal of Oncology 37.2 (2010): 273-279.
Chicago
Yamaoka, Kawasaki, Xu, Yamamoto, Terada, Okamura, and Shuji Kubo. "Establishment of in vivo fluorescence imaging in mouse models of malignant mesothelioma." International Journal of Oncology International Journal of Oncology 37 no. 2 (2010): 273-279.