Functional characterization of AIBp, a novel Aurora-A binding protein in centrosome structure and spindle formation

  • Authors: Ann-Shung Lieu, Tai-Shan Cheng, Chia-Hua Chou, Chia-Hung Wu, Chia-Yi Hsu, Chi-Ying F. Huang, Li-Kwan Chang, Joon-Khim Loh, Chung-Shing Chang, Ching-Mei Hsu, Shen-Long Howng, Yi-Ren Hong
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  • Published online on: Sunday, August 1, 2010
  • Pages: 429-436
  • DOI: 10.3892/ijo_00000691

Abstract

Aurora-A is involved in chromosome alignment, centrosome maturation, mitotic spindle assembly and regards to an oncogene. Aurora-A is also known to bind to several other proteins affecting its up-regulation or down-regulation and localization. However, how these different binding signals work together to regulate Aurora-A is not properly known. To explore more Aurora-A interacting proteins, the low-copy yeast two-hybrid screening using Aurora-A as bait protein was performed. One novel gene, AIBp, was demonstrated to associate with Aurora-A by the yeast two-hybrid method and in vitro GST pull-down assay. Molecular characterization showed that AIBp possessed a binding site at the C-terminal with Aurora-A (kinase domain). Interestingly, AIBp also interacts with hNinein at the N-terminal, which overlaps with a previously reported hNinein and GSK3β binding site. Using a kinase assay, AIBp interacts with the Aurora-A kinase domain functions as a positive regulator, whereas AIBp binding to hNinein appears to block the phosphorylation of hNinein by both Aurora-A and GSK3β. siRNA-mediated elimination of AIBp from HeLa cells, results in a doughnut-like shape, asymmetrical spindle pole and multiple spindle pole formation. We also demonstrated that both AIBp and Aurora-A are co-overexpressed in various brain tumors. These studies demonstrate that AIBp may not only be required for the dynamic movement of Aurora-A at the centrosomes and spindle apparatus during the cell cycle, but may also be important during brain tumorigenesis.
Journal Cover

August 2010
Volume 37 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

2013 Impact Factor: 2.773
Ranked #30/202 Oncology
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APA
Lieu, A., Cheng, T., Chou, C., Wu, C., Hsu, C., Huang, C., Chang, L., Loh, J., Chang, C., Hsu, C., Howng, S., & Hong, Y. (2010). Functional characterization of AIBp, a novel Aurora-A binding protein in centrosome structure and spindle formation. International Journal of Oncology, 37(2), 429-436.
MLA
Lieu, Cheng, Chou, Wu, Hsu, Huang, Chang, Loh, Chang, Hsu, Howng, and Yi-Ren Hong. "Functional characterization of AIBp, a novel Aurora-A binding protein in centrosome structure and spindle formation." International Journal of Oncology International Journal of Oncology 37.2 (2010): 429-436.
Chicago
Lieu, Cheng, Chou, Wu, Hsu, Huang, Chang, Loh, Chang, Hsu, Howng, and Yi-Ren Hong. "Functional characterization of AIBp, a novel Aurora-A binding protein in centrosome structure and spindle formation." International Journal of Oncology International Journal of Oncology 37 no. 2 (2010): 429-436.