Characterisation of a multimeric protein complex associated with ERp57 within the nucleus in paclitaxel-sensitive and -resistant epithelial ovarian cancer cells: The involvement of specific conformational states of β-actin

  • Authors: Lucia Cicchillitti, Anna Della Corte, Michela Di Michele, Maria Benedetta Donati, Domenico Rotilio, Giovanni Scambia
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  • Published online on: Sunday, August 1, 2010
  • Pages: 445-454
  • DOI: 10.3892/ijo_00000693

Abstract

Ovarian cancer is the second most frequently diagnosed malignancy of the reproductive system and is the leading cause of gynecological cancer mortality. Although the majority of advanced ovarian carcinomas initially respond successfully to taxane-based chemotherapy, resistance to chemotherapy remains the primary factor accounting for the low 5-year survival in this patient population. Recent data obtained by our group demonstrate that the disulphide isomerase ERp57 is strongly modulated in paclitaxel resistance suggesting that it may represent a chemoresistance biomarker in ovarian cancer. In the present study, we characterise a nuclear multimeric complex where ERp57 is associated with protein species involved in cell division and gene expression, as Nucleolin, Nucleophosmin, Vimentin, Aurora kinase C and β-actin. In particular, we show that the occurrence of the interaction of nuclear ERp57 with β-actin is associated with paclitaxel resistance and that specific actin conformations modulate this complex. We propose the involvement of the nuclear ERp57 complex in mechanisms associated with chromosome segregation in which specific conformational states of actin play a role in the pathway involved in paclitaxel resistance.
Journal Cover

August 2010
Volume 37 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

2013 Impact Factor: 2.773
Ranked #30/202 Oncology
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APA
Cicchillitti, L., Della Corte, A., Di Michele, M., Donati, M., Rotilio, D., & Scambia, G. (2010). Characterisation of a multimeric protein complex associated with ERp57 within the nucleus in paclitaxel-sensitive and -resistant epithelial ovarian cancer cells: The involvement of specific conformational states of β-actin. International Journal of Oncology, 37(2), 445-454.
MLA
Cicchillitti, Della Corte, Di Michele, Donati, Rotilio, and Giovanni Scambia. "Characterisation of a multimeric protein complex associated with ERp57 within the nucleus in paclitaxel-sensitive and -resistant epithelial ovarian cancer cells: The involvement of specific conformational states of β-actin." International Journal of Oncology International Journal of Oncology 37.2 (2010): 445-454.
Chicago
Cicchillitti, Della Corte, Di Michele, Donati, Rotilio, and Giovanni Scambia. "Characterisation of a multimeric protein complex associated with ERp57 within the nucleus in paclitaxel-sensitive and -resistant epithelial ovarian cancer cells: The involvement of specific conformational states of β-actin." International Journal of Oncology International Journal of Oncology 37 no. 2 (2010): 445-454.