Mitotane sensitizes adrenocortical cancer cells to ionizing radiations by involvement of the cyclin B1/CDK complex in G2 arrest and mismatch repair enzymes modulation

  • Authors:
    • Lidia Cerquetti
    • Camilla Sampaoli
    • Donatella Amendola
    • Barbara Bucci
    • Silvia Misiti
    • Giorgio Raza
    • Ugo De Paula
    • Rodolfo Marchese
    • Ercole Brunetti
    • Vincenzo Toscano
    • Antonio Stigliano
  • View Affiliations

  • Published online on: Sunday, August 1, 2010
  • Pages: 493-501
  • DOI: 10.3892/ijo_00000698

Abstract

Mitotane inhibits steroid synthesis by an action on steroidogenic enzymes, as 11β-hydroxylase and cholesterol side chain cleavage. It also has a cytotoxic effect on the adrenocortical cells and represents a primary drug used in the adrenocortical carcinoma (ACC). H295R and SW13 cell lines were treated with mitotane 10−5 M and ionizing radiations (IR) in combination therapy, inducing an irreversible inhibition of cell growth in both adrenocortical cancer cells. As shown in a previous report, mitotane/IR combination treatment induced a cell accumulation in the G2 phase. Here, we report the radiosensitizing properties of mitotane in two different ACC cell lines. The drug reveals the effectiveness to enhance the cytotoxic effects of IR by attenuating DNA repair and interfering on the activation of mitosis promoting factor (MPF), mainly regulated by the degradation of cyclin B1 in the mitotic process. These events may explain the inappropriate activation of cdc2, implicated in G2/M phase arrest and probably induced by the mitotane and IR in the combined treatment. Indeed, treatment with purvalanol, a cdc2-inhibitor prevents cell cycle arrest, triggering the G2/M transition. The observation that mitotane and IR in combination treatment amplifies the activation level of cyclin B/cdc2 complexes contributing to cell cycle arrest, suggests that the MPF could function as a master signal for controlling the temporal order of different mitotic events. Moreover, we report that mitotane interferes in modulation of mismatch repair (MMR) enzymes, revealing radiosensitizing drug ability.
Journal Cover

August 2010
Volume 37 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

2013 Impact Factor: 2.773
Ranked #30/202 Oncology
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APA
Cerquetti, L., Sampaoli, C., Amendola, D., Bucci, B., Misiti, S., Raza, G., De Paula, U., Marchese, R., Brunetti, E., Toscano, V., & Stigliano, A. (2010). Mitotane sensitizes adrenocortical cancer cells to ionizing radiations by involvement of the cyclin B1/CDK complex in G2 arrest and mismatch repair enzymes modulation. International Journal of Oncology, 37(2), 493-501.
MLA
Cerquetti, Sampaoli, Amendola, Bucci, Misiti, Raza, De Paula, Marchese, Brunetti, Toscano, and Antonio Stigliano. "Mitotane sensitizes adrenocortical cancer cells to ionizing radiations by involvement of the cyclin B1/CDK complex in G2 arrest and mismatch repair enzymes modulation." International Journal of Oncology International Journal of Oncology 37.2 (2010): 493-501.
Chicago
Cerquetti, Sampaoli, Amendola, Bucci, Misiti, Raza, De Paula, Marchese, Brunetti, Toscano, and Antonio Stigliano. "Mitotane sensitizes adrenocortical cancer cells to ionizing radiations by involvement of the cyclin B1/CDK complex in G2 arrest and mismatch repair enzymes modulation." International Journal of Oncology International Journal of Oncology 37 no. 2 (2010): 493-501.