Ganodermanontriol, a lanostanoid triterpene from Ganoderma lucidum, suppresses growth of colon cancer cells through ß-catenin signaling

  • Authors:
    • Andrej Jedinak
    • Anita Thyagarajan-Sahu
    • Jiahua Jiang
    • Daniel Sliva
  • View Affiliations

  • Published online on: Tuesday, January 11, 2011
  • Pages: 761-767
  • DOI: 10.3892/ijo.2011.898

Abstract

Colorectal cancer is one of the most common cancers in men and women in the world. Previous molecular studies have revealed that deregulation of the ß-catenin signaling pathway plays a crucial role in the progression of colorectal cancer. Therefore, modulation of the ß-catenin pathway offers a strategy to control colorectal cancer progression. The medicinal mushroom Ganoderma lucidum (GL) is a rich source of triterpenes with anticancer properties. Here, we show that ganodermanontriol (GNDT), a purified triterpene from GL, inhibited proliferation of HCT-116 and HT-29 colon cancer cells without a significant effect on cell viability. Moreover, GNDT inhibited transcriptional activity of ß-catenin and protein expression of its target gene cyclin D1 in a dose-dependent manner. A marked inhibition effect was also seen on Cdk-4 and PCNA expression, whereas expression of Cdk-2, p21 and cyclin E was not affected by the GNDT treatment. In addition, GNDT caused a dose-dependent increase in protein expression of E-cadherin and ß-catenin in HT-29 cells. Finally, GNDT suppressed tumor growth in a xenograft model of human colon adenocarcinoma cells HT-29 implanted in nude mice without any side-effects and inhibited expression of cyclin D1 in tumors. In conclusion, our data suggest that ganodermanontriol might be a potential chemotherapeutic agent for the treatment of cancer.
Journal Cover

March 2011
Volume 38 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

2013 Impact Factor: 2.773
Ranked #30/202 Oncology
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APA
Jedinak, A., Thyagarajan-Sahu, A., Jiang, J., & Sliva, D. (2011). Ganodermanontriol, a lanostanoid triterpene from Ganoderma lucidum, suppresses growth of colon cancer cells through ß-catenin signaling. International Journal of Oncology, 38(3), 761-767.
MLA
Jedinak, Thyagarajan-Sahu, Jiang, and Daniel Sliva. "Ganodermanontriol, a lanostanoid triterpene from Ganoderma lucidum, suppresses growth of colon cancer cells through ß-catenin signaling." International Journal of Oncology International Journal of Oncology 38.3 (2011): 761-767.
Chicago
Jedinak, Thyagarajan-Sahu, Jiang, and Daniel Sliva. "Ganodermanontriol, a lanostanoid triterpene from Ganoderma lucidum, suppresses growth of colon cancer cells through ß-catenin signaling." International Journal of Oncology International Journal of Oncology 38 no. 3 (2011): 761-767.