TNF-α is a novel target of miR-19a

  • Authors: Mei Liu, Zaozao Wang, Shangbin Yang, Wei Zhang, Shun He, Chenfei Hu, Hongxia Zhu, Lanping Quan, Jinfeng Bai, Ningzhi Xu
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  • Published online on: Tuesday, January 25, 2011
  • Pages: 1013-1022
  • DOI: 10.3892/ijo.2011.924

Abstract

Many studies have demonstrated the overexpression and amplification of the miR-17-92 cluster in malignant human cancers, including B-cell lymphomas and lung cancers. The purpose of this study was to investigate for the first time, the expression of the miR-17-92 cluster in esophageal squamous cell carcinoma (ESCC). The miR-17-92 cluster was found to be overexpressed in 21 out of 28 (75%) esophageal cancer samples. It was also found that overexpression of the miR-17-92 cluster could promote cellular growth in vivo and in vitro. Furthermore, inhibition of miR-19a by antisense oligonucleotides (ONs) induced apoptosis, while antisense ONs against miR-17-5p, miR-18a, miR-20a and miR-92-1 did not exhibit such an effect. In addition, it was found that antagomir-19a treatment could impair tumor growth in vivo. Using Human Apoptosis RT2 Profiler PCR Array 384HT, we found that tumor necrosis factor-α (TNF-α) was up-regulated 12-fold in cells transfected with miR-19a antisense ONs compared to the cells treated with the control scramble ONs. MiR-19a was predicted to target the 3' untranslated region of TNF-α mRNA, and this was confirmed by luciferase reporter assay. Taken together, we conclude that the miR-17-92 cluster is overexpressed in ESCC and that TNF-α is a novel target of miR-19a.
Journal Cover

April 2011
Volume 38 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

2012 Impact Factor: 2.773
Ranked #31/196 Oncology
(total number of cites)

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APA
Liu, M., Wang, Z., Yang, S., Zhang, W., He, S., Hu, C., Zhu, H., Quan, L., Bai, J., & Xu, N. (2011). TNF-α is a novel target of miR-19a. International Journal of Oncology, 38(4), 1013-1022.
MLA
Liu, Wang, Yang, Zhang, He, Hu, Zhu, Quan, Bai, and Ningzhi Xu. "TNF-α is a novel target of miR-19a." International Journal of Oncology International Journal of Oncology 38.4 (2011): 1013-1022.
Chicago
Liu, Wang, Yang, Zhang, He, Hu, Zhu, Quan, Bai, and Ningzhi Xu. "TNF-α is a novel target of miR-19a." International Journal of Oncology International Journal of Oncology 38 no. 4 (2011): 1013-1022.