Open Access

Combination effects of distinct cores in 11q13 amplification region on cervical lymph node metastasis of oral squamous cell carcinoma

  • Authors:
    • Keisuke Sugahara
    • Yuichi Michikawa
    • Kenichi Ishikawa
    • Yoshimi Shoji
    • Mayumi Iwakawa
    • Takahiko Shibahara
    • Takashi Imai
  • View Affiliations

  • Published online on: Wednesday, June 22, 2011
  • Pages: 761-769
  • DOI: 10.3892/ijo.2011.1094

Abstract

Lymph node metastasis (LNM) in oral squamous cell carcinoma (OSCC) is known to associate with a significant decrease of 5-year survival. Genetic factors related to the difference of the LNM status in the OSCC have been not fully elucidated. Array-based comparative genomic hybridization (CGH) with individual gene-level resolution and real-time quantitative polymerase chain reaction (QPCR) were conducted using primary tumor materials resected from 54 OSCC patients with (n=22) or without (n=32) cervical LNM. Frequent gain was observed at the 11q13 region exclusively in patients with cervical LNM, which was confirmed by real-time QPCR experiments using 11 genes (TPCN2, MYEOV, CCND1, ORAOV1, FGF4, TMEM16A, FADD, PPFIA1, CTTN, SHANK2 and DHCR7) in this region. It was revealed that two distinct amplification cores existed, which were separated by a breakpoint between MYEOV and CCND1 in the 11q13 region. The combination of copy number amplification at CTTN (core 2) and/or TPCN2/MYEOV (core 1), selected from each core, was most significantly associated with cervical LNM (P=0.0035). Two amplification cores at the 11q13 region may have biological impacts on OSCC cells to spread from the primary site to local lymph nodes. Further study of a larger patient series should be conducted to validate these results.
Journal Cover

October 2011
Volume 39 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

2013 Impact Factor: 2.773
Ranked #30/202 Oncology
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APA
Sugahara, K., Michikawa, Y., Ishikawa, K., Shoji, Y., Iwakawa, M., Shibahara, T., & Imai, T. (2011). Combination effects of distinct cores in 11q13 amplification region on cervical lymph node metastasis of oral squamous cell carcinoma. International Journal of Oncology, 39(4), 761-769.
MLA
Sugahara, Michikawa, Ishikawa, Shoji, Iwakawa, Shibahara, and Takashi Imai. "Combination effects of distinct cores in 11q13 amplification region on cervical lymph node metastasis of oral squamous cell carcinoma." International Journal of Oncology International Journal of Oncology 39.4 (2011): 761-769.
Chicago
Sugahara, Michikawa, Ishikawa, Shoji, Iwakawa, Shibahara, and Takashi Imai. "Combination effects of distinct cores in 11q13 amplification region on cervical lymph node metastasis of oral squamous cell carcinoma." International Journal of Oncology International Journal of Oncology 39 no. 4 (2011): 761-769.