Rac1b recruits Dishevelled and β-catenin to Wnt target gene promoters independent of Wnt3A stimulation

  • Authors: Vaijayanti V. Pethe, George S. Charames, Bharati Bapat
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  • Published online on: Monday, June 6, 2011
  • Pages: 805-810
  • DOI: 10.3892/ijo.2011.1066

Abstract

We previously reported a functional interaction between aberrant Wnt signaling and Rac1/Rac1b GTPases in tumorigenesis. In this study, we further investigated the mechanistic role of nuclear Rac1b. Using chromatin immunoprecipitation (ChIP) studies, we show that Rac1b resides at the promoters of Wnt target genes, c-Myc and Cyclin D1, in HCT116 cells with aberrant Wnt pathway. In HEK293T cells with intact Wnt signaling, Rac1b is tethered to these same gene promoters independent of Wnt3A stimulation and is further observed to recruit Dishevelled and β-catenin in the absence of Wnt3A stimulation. Our studies suggest a novel transcriptional co-activator role of Rac1b in β-catenin/TCF-mediated transcription.
Journal Cover

October 2011
Volume 39 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

2012 Impact Factor: 2.773
Ranked #30/202 Oncology
(total number of cites)

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APA
Pethe, V., Charames, G., & Bapat, B. (2011). Rac1b recruits Dishevelled and β-catenin to Wnt target gene promoters independent of Wnt3A stimulation. International Journal of Oncology, 39(4), 805-810.
MLA
Pethe, Charames, and Bharati Bapat. "Rac1b recruits Dishevelled and β-catenin to Wnt target gene promoters independent of Wnt3A stimulation." International Journal of Oncology International Journal of Oncology 39.4 (2011): 805-810.
Chicago
Pethe, Charames, and Bharati Bapat. "Rac1b recruits Dishevelled and β-catenin to Wnt target gene promoters independent of Wnt3A stimulation." International Journal of Oncology International Journal of Oncology 39 no. 4 (2011): 805-810.