Induction of specific human cytotoxic T cells using dendritic cells transduced with an adenovector encoding rat epidermal growth factor receptor 2

  • Authors:
    • Kalathil Suresh
    • Elizabeth Scheid
    • Laurence Klotz
    • Vasundara Venkateswaran
    • Jack Gauldie
    • Ronan Foley
  • View Affiliations

  • Published online on: Thursday, July 14, 2011
  • Pages: 907-913
  • DOI: 10.3892/ijo.2011.1124

Abstract

This study demonstrates the ability to generate antigen-specific cytotoxic T cells (CTLs) against HER2 using a xenoantigenic immune stimulation strategy. Dendritic cells (DCs) were transduced with an adenovirus vector incorporating full-length cDNA for rat (xenoantigen) epidermal growth factor receptor 2 (Adv-HER2). Stimulation of autologous T cells with Adv-HER2 infected DCs led to enhanced HER2-specific reactivity as assessed by quantitative real-time polymerase chain reaction (qRT-PCR) for T cell IFN-γ mRNA. In ELISPOT and intracellular cytokine staining (ICS) assays, CD8+ CTLs induced by Adv-HER2 transduced DCs released IFN-γ following stimulation with irradiated autologous DCs infected with Adv-HER2 or loaded with a human prostate cancer cell line (LNCaP) lysate. DCs pulsed with HER2 peptides were less stimulatory than Adv-HER2 transduced DCs. HER2 DC induced CTL lysed HER2+ HLA-A2+ tumor cells (MCF-7); significantly reduced lysis occurred in HER2+ HLA-A2- tumor cells (SKOV-3), and the NK cell sensitive cell line K-562.
Journal Cover

October 2011
Volume 39 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

2013 Impact Factor: 2.773
Ranked #30/202 Oncology
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APA
Suresh, K., Scheid, E., Klotz, L., Venkateswaran, V., Gauldie, J., & Foley, R. (2011). Induction of specific human cytotoxic T cells using dendritic cells transduced with an adenovector encoding rat epidermal growth factor receptor 2. International Journal of Oncology, 39(4), 907-913.
MLA
Suresh, Scheid, Klotz, Venkateswaran, Gauldie, and Ronan Foley. "Induction of specific human cytotoxic T cells using dendritic cells transduced with an adenovector encoding rat epidermal growth factor receptor 2." International Journal of Oncology International Journal of Oncology 39.4 (2011): 907-913.
Chicago
Suresh, Scheid, Klotz, Venkateswaran, Gauldie, and Ronan Foley. "Induction of specific human cytotoxic T cells using dendritic cells transduced with an adenovector encoding rat epidermal growth factor receptor 2." International Journal of Oncology International Journal of Oncology 39 no. 4 (2011): 907-913.