P-glycoprotein and survivin simultaneously regulate vincristine-induced apoptosis in chronic myeloid leukemia cells

  • Authors:
    • Paloma Silva Souza
    • Flavia Cunha Vasconcelos
    • Flaviana Ruade De Souza Reis
    • Gabriela Nestal De Moraes
    • Raquel Ciuvalschi Maia
  • View Affiliations

  • Published online on: Tuesday, June 28, 2011
  • Pages: 925-933
  • DOI: 10.3892/ijo.2011.1103

Abstract

Overexpression of P-glycoprotein (Pgp/ABCB1) in tumor cells is associated with a classic phenotype of multidrug resistance (MDR). Moreover, some members of the inhibitor of apoptosis protein (IAP) family, such as survivin, contribute to an apoptosis-resistant phenotype, by inhibiting chemotherapy-induced cell death and promoting MDR. By using Western blotting, qRT-PCR, Annexin V and immunofluorescence assays we have demonstrated a relationship between Pgp and survivin in a prior sensitive chronic myeloid leukemia (CML) cell line (K562). A high dose of vincristine induced a concomitant overexpression of Pgp and survivin, which was associated with a low apoptotic index in the K562 cell line. In addition, we observed a cytoplasmic co-localization of Pgp and survivin, suggesting a functional association between these two proteins in apoptosis control by a common mechanism. In summary, our data suggest that Pgp and survivin should be analyzed in aggregate because they may have significant impact on drug resistance in CML cells.
Journal Cover

October 2011
Volume 39 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

2013 Impact Factor: 2.773
Ranked #30/202 Oncology
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APA
Souza, P., Vasconcelos, F., De Souza Reis, F., De Moraes, G., & Maia, R. (2011). P-glycoprotein and survivin simultaneously regulate vincristine-induced apoptosis in chronic myeloid leukemia cells. International Journal of Oncology, 39(4), 925-933.
MLA
Souza, Vasconcelos, De Souza Reis, De Moraes, and Raquel Maia. "P-glycoprotein and survivin simultaneously regulate vincristine-induced apoptosis in chronic myeloid leukemia cells." International Journal of Oncology International Journal of Oncology 39.4 (2011): 925-933.
Chicago
Souza, Vasconcelos, De Souza Reis, De Moraes, and Raquel Maia. "P-glycoprotein and survivin simultaneously regulate vincristine-induced apoptosis in chronic myeloid leukemia cells." International Journal of Oncology International Journal of Oncology 39 no. 4 (2011): 925-933.