Establishment and detailed functional and molecular genetic characterisation of a novel sacral chordoma cell line, MUG-Chor1

  • Authors:
    • Beate Rinner
    • Elke Verena Froehlich
    • Karin Buerger
    • Heike Knausz
    • Birgit  Lohberger
    • Susanne Scheipl
    • Carina Fischer
    • Andreas Leithner
    • Christian  Guelly
    • Slave Trajanoski
    • Karoly Szuhai
    • Bernadette Liegl
  • View Affiliations

  • Published online on: Friday, October 14, 2011
  • Pages: 443-451
  • DOI: 10.3892/ijo.2011.1235

Abstract

Chordomas are rare, low to intermediate grade malignant bone tumors of the axial skeleton. Current treatment options are limited to surgical procedures, as chordomas are largely resistant to conventional radiation and chemotherapy. Cell lines are valuable tools for exploring molecular mechan­isms involved in tumorigenesis and they have a fundamental impact on the development of new anticancer agents. To date, only two chordoma cell lines exist world-wide. In the present study we report a third chordoma cell line, MUG-Chor1, as well as corresponding cultured fibroblasts established from a recur­rent morphologically ‘classic’ sacrococcygeal chordoma of a 58-year-old Caucasian female. The cells are brachyury-positive and have the characteristics of chordoma. The genetic profile of the primary chordoma and the established chordoma cell line was investigated during the culturing period (early and late passage). MUG-Chor1 is karyotypically, <2n>43-47,XX,del(3)(q1?)[11], +7,del(9)(p1?),der(9;15)(q10;q10),-10,+der(12)t(9;12)(p2?;q1?),der (12)t(12;19)(p;p)t(17;19)(q;q),-15,der(17;21)(q10;q10),der(20)t(10;20) (q25?26?;q11?12?),-21,-22[20]/idemx2[5] and displays known, chordoma-typical genetic changes, such as chromosomal gains at T/brachyury locus (6q27), losses at 9p24.3-p13.1 (includes the CDKN2a/CDKN2b locus), 10p15.3-q23.32 (includes the PTEN locus) and losses of 10q25.2 (includes the PDCD4 locus). MUG-Chor1 bears a marked resemblance to chordomas in vivo and is, therefore, an optimal in vitro chordoma model.
Journal Cover

February 2012
Volume 40 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

2013 Impact Factor: 2.773
Ranked #30/202 Oncology
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APA
Rinner, B., Rinner, B., Froehlich, E., Froehlich, E., Buerger, K., Buerger, K., Knausz, H., Knausz, H., Lohberger, B., Lohberger, B., Scheipl, S., Scheipl, S., Fischer, C., Fischer, C., Leithner, A., Leithner, A., Guelly, C., Guelly, C., Trajanoski, S., Trajanoski, S., Szuhai, K., Szuhai, K., Liegl, B., & Liegl, B. (2012). Establishment and detailed functional and molecular genetic characterisation of a novel sacral chordoma cell line, MUG-Chor1. International Journal of Oncology, 40(2), 443-451.
MLA
Rinner, Rinner, Froehlich, Froehlich, Buerger, Buerger, Knausz, Knausz, Lohberger, Lohberger, Scheipl, Scheipl, Fischer, Fischer, Leithner, Leithner, Guelly, Guelly, Trajanoski, Trajanoski, Szuhai, Szuhai, Liegl, and Bernadette Liegl. "Establishment and detailed functional and molecular genetic characterisation of a novel sacral chordoma cell line, MUG-Chor1." International Journal of Oncology International Journal of Oncology 40.2 (2012): 443-451.
Chicago
Rinner, Rinner, Froehlich, Froehlich, Buerger, Buerger, Knausz, Knausz, Lohberger, Lohberger, Scheipl, Scheipl, Fischer, Fischer, Leithner, Leithner, Guelly, Guelly, Trajanoski, Trajanoski, Szuhai, Szuhai, Liegl, and Bernadette Liegl. "Establishment and detailed functional and molecular genetic characterisation of a novel sacral chordoma cell line, MUG-Chor1." International Journal of Oncology International Journal of Oncology 40 no. 2 (2012): 443-451.