Identification of a long non-coding RNA gene, growth hormone secretagogue receptor opposite strand, which stimulates cell migration in non-small cell lung cancer cell lines

Whiteside,Eliza  J.; Seim,Inge; Pauli,Jana  P.; O'Keeffe,Angela   J.; Thomas,Patrick   B.; Carter,Shea  L.; Walpole,Carina  M.; Fung,Jenny  N.T.; Josh,Peter; Herington,Adrian  C.; Chopin,Lisa  K.

doi:10.3892/ijo.2013.1969

Identification of a long non-coding RNA gene, growth hormone secretagogue receptor opposite strand, which stimulates cell migration in non-small cell lung cancer cell lines

Authors:
- Eliza J. Whiteside
- Inge Seim
- Jana P. Pauli
- Angela J. O'Keeffe
- Patrick B. Thomas
- Shea L. Carter
- Carina M. Walpole
- Jenny N.T. Fung
- Peter Josh
- Adrian C. Herington
- Lisa K. Chopin
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Affiliations: Ghrelin Research Group, Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Queensland, Australia, Department of Physiology and Pharmacology, School of Biomedical Sciences, University of Queensland, Brisbane, Queensland, Australia, Commonwealth Scientific and Industrial Research Organisation, Brisbane, Queensland, Australia
Published online on: May 30, 2013 https://doi.org/10.3892/ijo.2013.1969
Pages: 566-574

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Abstract

The molecular mechanisms involved in non‑small cell lung cancer tumourigenesis are largely unknown; however, recent studies have suggested that long non-coding RNAs (lncRNAs) are likely to play a role. In this study, we used public databases to identify an mRNA-like, candidate long non-coding RNA, GHSROS (GHSR opposite strand), transcribed from the antisense strand of the ghrelin receptor gene, growth hormone secretagogue receptor (GHSR). Quantitative real-time RT-PCR revealed higher expression of GHSROS in lung cancer tissue compared to adjacent, non-tumour lung tissue. In common with many long non-coding RNAs, GHSROS is 5' capped and 3' polyadenylated (mRNA-like), lacks an extensive open reading frame and harbours a transposable element. Engineered overexpression of GHSROS stimulated cell migration in the A549 and NCI-H1299 non-small cell lung cancer cell lines, but suppressed cell migration in the Beas-2B normal lung-derived bronchoepithelial cell line. This suggests that GHSROS function may be dependent on the oncogenic context. The identification of GHSROS, which is expressed in lung cancer and stimulates cell migration in lung cancer cell lines, contributes to the growing number of non-coding RNAs that play a role in the regulation of tumourigenesis and metastatic cancer progression.

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